Li Xiang, Sun Yan, Liu Junjie, Yao Qin, Wang Guanghua
Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, China.
Graduate University of Chinese Academy of Sciences, Beijing, 100039, China.
Curr Microbiol. 2019 Jul;76(7):863-871. doi: 10.1007/s00284-019-01700-0. Epub 2019 May 8.
Although bacteriophages are the most abundant biological entities on the planet, their genetic diversity, especially in natural wetlands, is poorly understood. In this study, the genetic diversity of cyanopodoviruses in sediments of two coastal wetlands in Northeast China was investigated by targeting the DNA polymerase (pol) gene. A total of 66 DNA pol clones were obtained. A BLAST search at the amino acid level showed that the obtained sequences had the highest identity ranged from 83 to 99% to the known sequences. A phylogenetic tree showed that the distribution patterns of DNA pol sequence were different between two wetland soils, and 29 clones of this study formed four wetland-specific groups, which suggested that unrevealed novel groups of cyanopodovirus inhabited in wetlands. In addition, nonmetric multidimensional scaling (NMDS) analysis of all DNA pol sequences from various environments showed that cyanopodovirus communities of coastal wetlands are in the intermediate position between marine water environments and terrestrial freshwater environments, which highlights that the coastal wetlands as transitional zones between inland freshwater environments and marine environments.
尽管噬菌体是地球上数量最为丰富的生物实体，但其遗传多样性，尤其是在天然湿地中的遗传多样性，仍鲜为人知。在本研究中，通过靶向DNA聚合酶（pol）基因，对中国东北两个沿海湿地沉积物中的蓝藻短尾病毒的遗传多样性进行了调查。共获得了66个DNA pol克隆。氨基酸水平的BLAST搜索显示，获得的序列与已知序列的最高同源性在83%至99%之间。系统发育树表明，两个湿地土壤中DNA pol序列的分布模式不同，本研究中的29个克隆形成了四个湿地特异性类群，这表明湿地中存在未被揭示的新型蓝藻短尾病毒类群。此外，对来自各种环境的所有DNA pol序列进行的非度量多维尺度分析（NMDS）表明，沿海湿地的蓝藻短尾病毒群落处于海洋水环境和陆地淡水环境之间的中间位置，这突出了沿海湿地作为内陆淡水环境和海洋环境之间的过渡带。
