Bambino Kathryn, Morrison Joshua, Chu Jaime
Department of Environmental Medicine and Public Health, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Methods Mol Biol. 2019;1965:129-138. doi: 10.1007/978-1-4939-9182-2_9.
Zebrafish (Danio rerio) larvae are a uniquely powerful model system which investigate the effects of toxicant exposure on liver development and function. Manufacturing processes and development of new synthetic compounds increased rapidly since the middle of the twentieth century, resulting in widespread exposure to environmental toxicants. This is compounded by the shift in the global burden of disease from infectious agents to chronic disease, particularly in industrialized nations, which increases the need to investigate the long-term and transgenerational effects of environmental exposures on human health. Zebrafish provide an excellent model to investigate the mechanisms of action of environmental pollutants given their large-scale embryo production and rapid development, which allow for short-term assessment of toxicity in a whole animal system. Here we describe methods for the use of zebrafish to study hepatotoxicity and liver disease induced by chemical toxicants. Many of the genetic, molecular, and cellular processes are conserved between zebrafish and mammals, enabling translation to human populations and diseases.
斑马鱼（Danio rerio）幼体是一种独特且强大的模型系统，可用于研究接触有毒物质对肝脏发育和功能的影响。自20世纪中叶以来，新合成化合物的制造工艺和开发迅速发展，导致环境有毒物质广泛存在。全球疾病负担从传染病向慢性病的转变使这一情况更加复杂，尤其是在工业化国家，这增加了研究环境暴露对人类健康的长期和跨代影响的必要性。鉴于斑马鱼能够大规模生产胚胎且发育迅速，可在全动物系统中进行短期毒性评估，因此它为研究环境污染物的作用机制提供了一个极佳的模型。在此，我们描述了利用斑马鱼研究化学毒物诱导的肝毒性和肝病的方法。斑马鱼与哺乳动物之间许多遗传、分子和细胞过程是保守的，这使得研究结果能够转化应用于人类群体和疾病。
