利用斑马鱼正向遗传筛选鉴定与毒物相互作用的基因

Identifying Toxicant-Interacting Genes Using Forward Genetic Screening in Zebrafish.

作者信息

Hill Jonathon T

机构信息

Department of Physiology and Developmental Biology, College of Life Sciences, Brigham Young University, Provo, UT, USA.

出版信息

Methods Mol Biol. 2019;1965:251-259. doi: 10.1007/978-1-4939-9182-2_17.

DOI:10.1007/978-1-4939-9182-2_17

PMID:31069680

Abstract

Forward genetic screening is an extremely powerful method for identifying novel genes driving a broad range of phenotypes. This protocol describes the complete process for conducting a forward genetic screen in zebrafish, including mutagenesis with N-ethyl-N-nitrosourea (ENU), mating, phenotypic screening, and genetic mapping.

摘要

正向遗传学筛选是鉴定驱动多种表型的新基因的一种极其强大的方法。本方案描述了在斑马鱼中进行正向遗传学筛选的完整过程，包括用N-乙基-N-亚硝基脲（ENU）诱变、交配、表型筛选和基因定位。