Ilisz István, Bajtai Attila, Péter Antal, Lindner Wolfgang
Institute of Pharmaceutical Analysis, University of Szeged, Szeged, Hungary.
Department of Analytical Chemistry, University of Vienna, Vienna, Austria.
Methods Mol Biol. 2019;1985:251-277. doi: 10.1007/978-1-4939-9438-0_14.
For the early 2000s, chromatographic methods applying chiral stationary phases (CSPs) became the most effective techniques for the resolution of chiral compounds on both analytical and preparative scales. High-performance liquid chromatography (HPLC) employing various types of chiral selectors covalently bonded to silica-based supports offers a state-of-the-art methodology for "chiral analysis." Although a large number of CSPs are available nowadays, the design and development of new "chiral columns" are still needed since it is obvious that in practice one needs a good portfolio of different columns to face the challenging task of enantiomeric resolutions. The development of the unique chiral anion, cation, and zwitterion exchangers achieved by Lindner and his partners serves as an expansion of the range of the efficiently applicable CSPs.In this context this overview chapter discusses and summarizes direct enantiomer separations of chiral acids and ampholytes applying zwitterionic ion exchangers derived from Cinchona alkaloids. Our aim is to provide comprehensive information on practical solutions with focus on the molecular recognition and methodological variables.
在21世纪初，应用手性固定相（CSPs）的色谱方法成为在分析和制备规模上拆分手性化合物的最有效技术。采用各种与硅胶基载体共价键合的手性选择剂的高效液相色谱（HPLC）为“手性分析”提供了一种先进的方法。尽管如今有大量的CSPs可供使用，但新“手性柱”的设计和开发仍然是必要的，因为在实际操作中显然需要一系列不同的柱子来应对对映体拆分这一具有挑战性的任务。Lindner及其合作伙伴开发的独特手性阴离子、阳离子和两性离子交换剂扩大了高效适用CSPs的范围。在此背景下，本章综述讨论并总结了应用源自金鸡纳生物碱的两性离子交换剂对手性酸和两性电解质进行直接对映体分离的情况。我们的目的是提供关于实际解决方案的全面信息，重点关注分子识别和方法变量。
