Wahl Joachim, Holzgrabe Ulrike
Institute of Pharmacy and Food Chemistry, University of Würzburg, Wuerzburg, Germany.
Methods Mol Biol. 2019;1985:365-371. doi: 10.1007/978-1-4939-9438-0_20.
For enantioseparations of chiral drugs in capillary electrophoresis, chiral ionic liquids (CIL) can be employed instead of traditional running buffer containing a chiral selector. CILs can be applied solely or in addition to the often used cyclodextrin derivatives. Here the separation of phenethylamines, especially of ephedrine, is described using tetrabutylammonium L-argininate (125 mM) in phosphate buffer (75 mM, pH 1.5) in addition to β-cyclodextrin (30 mM). Using this dual-chiral running buffer system ephedrine, pseudoephedrine and methylephedrine, but not norephedrine, could be easily resolved.
在毛细管电泳中进行手性药物的对映体分离时，可以使用手性离子液体（CIL）来替代含有手性选择剂的传统运行缓冲液。CIL可以单独使用，也可以与常用的环糊精衍生物一起使用。本文描述了在磷酸盐缓冲液（75 mM，pH 1.5）中使用L-精氨酸四丁铵（125 mM）以及β-环糊精（30 mM）对苯乙胺类，尤其是麻黄碱的分离。使用这种双手机性运行缓冲液系统，可以轻松分离麻黄碱、伪麻黄碱和甲基麻黄碱，但去甲麻黄碱无法分离。
