使用二硫醇反应性探针通过流式细胞术评估促凝血小板

Flow Cytometry Assessment of Procoagulant Platelets Using a Dithiol-Reactive Probe.

作者信息

Tan Chuen Wen, Bourcy Morgane, Pasalic Leonardo, Chen Vivien M

机构信息

Department of Haematology, Singapore General Hospital, Singapore, Singapore.

Platelet and Thrombosis Research Laboratory, ANZAC Research Institute, Sydney, NSW, Australia.

出版信息

Methods Mol Biol. 2019;1967:305-321. doi: 10.1007/978-1-4939-9187-7_20.

DOI:10.1007/978-1-4939-9187-7_20

PMID:31069780

Abstract

Flow cytometry assessment of platelets using the combination of GSAO [4-(N-(S-glutathionylacetyl)amino)phenylarsonous acid], a dithiol-reactive probe, and P-selectin, a platelet activation marker, is a novel and powerful assay in the identification and quantification of the procoagulant subpopulation of platelets that has the capacity to support thrombin generation. In this chapter, we provide the flow cytometry protocols aimed at the study of procoagulant platelets under resting and agonist-stimulated conditions in whole blood and washed platelets of both human and murine (mouse) samples.

摘要

使用二硫醇反应性探针GSAO [4-(N-(S-谷胱甘肽乙酰基)氨基)苯胂酸]和血小板活化标志物P-选择素的组合，通过流式细胞术评估血小板，是一种用于识别和定量具有支持凝血酶生成能力的促凝血小板亚群的新颖且强大的检测方法。在本章中，我们提供了流式细胞术方案，旨在研究人源和鼠源（小鼠）样本的全血及洗涤血小板在静息和激动剂刺激条件下的促凝血小板。