磷脂酶可能参与催乳素（PRL）对Nb2淋巴结淋巴瘤细胞的促有丝分裂作用。

Possible involvement of the phospholipases in the mitogenic actions of prolactin (PRL) on Nb2 node lymphoma cells.

作者信息

Ofenstein J P, Rillema J A

出版信息

Proc Soc Exp Biol Med. 1987 Jun;185(2):147-52. doi: 10.3181/00379727-185-42529.

Abstract

The possible role of the phospholipase enzymes in the prolactin stimulation of mitogenesis in Nb2 node lymphoma cells was investigated. Two phospholipase inhibitors including quinacrine and alpha-para-dibromoacetophenone (BPB) were employed. Quinacrine at concentrations of 1-5 microM attenuated the magnitude of the PRL stimulation of cell division; at concentrations of 10 microM and above quinacrine abolished the PRL response. BPB at concentrations of 1-10 microM also inhibited the mitogenic effect of PRL in a concentration response fashion. The polyunsaturated fatty acid arachidonic acid partially reversed the inhibitory effects of these drugs. In further studies, exogenously added phospholipase C at concentrations of 5-50 ng/ml was found to potentiate the mitogenic effect of prolactin when prolactin was employed at a concentration that evoked a half-maximal response. By itself, however, phospholipase C had no effect on the rate of cell division. Phospholipase A2 either by itself or in the presence of prolactin was without effect.

摘要

研究了磷脂酶在催乳素刺激Nb2结节淋巴瘤细胞发生有丝分裂过程中可能发挥的作用。使用了两种磷脂酶抑制剂，即奎纳克林和α-对二溴苯乙酮（BPB）。浓度为1-5微摩尔的奎纳克林减弱了催乳素对细胞分裂的刺激程度；浓度为10微摩尔及以上时，奎纳克林消除了催乳素反应。浓度为1-10微摩尔的BPB也以浓度反应方式抑制了催乳素的促有丝分裂作用。多不饱和脂肪酸花生四烯酸部分逆转了这些药物的抑制作用。在进一步的研究中，当以能引起半数最大反应的浓度使用催乳素时，发现外源添加浓度为5-50纳克/毫升的磷脂酶C可增强催乳素的促有丝分裂作用。然而，磷脂酶C本身对细胞分裂速率没有影响。磷脂酶A2单独或在催乳素存在的情况下均无作用。