Sciensano, Transversal activities in Applied Genomics (TAG), J. Wytsmanstraat 14, 1050, Brussels, Belgium.
National Food Chain Safety Office, Mester utca 81, H-1024, Budapest, Hungary.
Sci Rep. 2019 May 9;9(1):7141. doi: 10.1038/s41598-019-43463-5.
In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the characterization of their transgene flanking regions as well as unnatural associations of elements from their transgenic cassette.
为了对未经授权的基因改良矮牵牛进行特征分析，我们在欧洲市场上的几个疑似矮牵牛样本上应用了一种综合策略。更确切地说，通过在早期通过实时 PCR 筛选分析检测到的关键靶标上进行 DNA 行走锚定，产生了感兴趣的 DNA 片段，然后使用牛津纳米孔技术公司的 MinION 平台进行测序。通过对其转基因盒中外源元件的侧翼区域以及非自然关联的特征分析，证明了基因改良矮牵牛的存在。
