Thermal gels: a procedure for determination of heat-inactivation temperature of enzymes.
作者信息
Manjrekar J, Krishnan K S
出版信息
Anal Biochem. 1987 Feb 1;160(2):409-13. doi: 10.1016/0003-2697(87)90068-6.
PMID:3107422
Abstract
A facile procedure for the study of inactivation temperatures of enzymes that could be stained for activity after electrophoresis is described. This is particularly suitable for enzymes that have multiple electrophoretic forms. The results of studies on Drosophila dehydrogenases and acetylcholinesterase are presented. The procedure may be useful in the study of genetic variants.
摘要
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