Ezaki K, Ito N, Suzuki M, Maruyama F, Kojima H, Sobue R, Matsui T, Ino T, Hirano M
Gan To Kagaku Ryoho. 1987 May;14(5 Pt 1):1246-51.
Large granular lymphocytes (LGL) were obtained by Percoll density gradient centrifugation of peripheral mononuclear cells from 15 patients with hematological malignancies (10 acute leukemias and 5 non-Hodgkin's lymphomas). Five-hour 51Cr-release cytotoxic assay by LGL was performed against frozen autologous tumor cells in these patients. Mean percentage cytotoxicity by LGL was 5.6%, and this was enhanced to 15.0% by the addition of IFN-beta to the culture medium. A decrease of cytotoxicity was observed when LGL was treated with anti-Leu 11b antibody plus complement, or when LGL was pretreated with the unlabelled K562 as a competitive inhibition assay. The addition of monocytes also induced a decrease of cytotoxicity, suggesting that monocytes may act as a suppressive agent in autologous tumor cell killing by LGL.
通过对15例血液系统恶性肿瘤患者（10例急性白血病和5例非霍奇金淋巴瘤）的外周单个核细胞进行Percoll密度梯度离心，获得大颗粒淋巴细胞（LGL）。对这些患者的冷冻自体肿瘤细胞进行了LGL的5小时51Cr释放细胞毒性试验。LGL的平均细胞毒性百分比为5.6%，当在培养基中添加IFN-β时，该百分比提高到15.0%。当LGL用抗Leu 11b抗体加补体处理时，或当LGL用未标记的K562作为竞争性抑制试验进行预处理时，观察到细胞毒性降低。添加单核细胞也会导致细胞毒性降低，这表明单核细胞可能在LGL杀伤自体肿瘤细胞过程中起抑制作用。
