血管活性肠肽对离体牛肾上腺嗜铬细胞儿茶酚胺合成的刺激作用。蛋白激酶C可能参与其中。

Stimulation by vasoactive intestinal polypeptide of catecholamine synthesis in isolated bovine adrenal chromaffin cells. Possible involvement of protein kinase C.

作者信息

Houchi H, Oka M, Misbahuddin M, Morita K, Nakanishi A

出版信息

Biochem Pharmacol. 1987 May 1;36(9):1551-4. doi: 10.1016/0006-2952(87)90125-0.

Abstract

In isolated bovine adrenal medullary cells, vasoactive intestinal polypeptide (VIP) stimulated 14C-catecholamine synthesis from 14C-tyrosine, but not from 14C-DOPA. This stimulatory effect of VIP on 14C-catecholamine synthesis was not dependent upon extracellular Ca2+. VIP did not affect the intracellular cyclic AMP (cAMP) level. The stimulatory effect of VIP on 14C-catecholamine synthesis was additive with that of carbamylcholine, which was dependent upon extracellular Ca2+, but not with that of phorbol ester 12-O-tetradecanoyl phorbol 13-acetate (TPA), an activator of protein kinase C. Moreover, 1-(isoquinolinyl-sulfonyl)-2-methylpiperazine (H-7), an inhibitor of protein kinase C, inhibited not only TPA-stimulated, but also VIP-stimulated 14C-catecholamine synthesis from 14C-tyrosine. These results suggested that VIP stimulated catecholamine synthesis by activation of tyrosine hydroxylase and that protein kinase C was involved in this stimulatory mechanism.

摘要

在分离的牛肾上腺髓质细胞中，血管活性肠肽（VIP）刺激了从¹⁴C-酪氨酸合成¹⁴C-儿茶酚胺，但不刺激从¹⁴C-多巴合成¹⁴C-儿茶酚胺。VIP对¹⁴C-儿茶酚胺合成的这种刺激作用不依赖于细胞外Ca²⁺。VIP不影响细胞内环磷酸腺苷（cAMP）水平。VIP对¹⁴C-儿茶酚胺合成的刺激作用与氨甲酰胆碱的刺激作用相加，氨甲酰胆碱的刺激作用依赖于细胞外Ca²⁺，但与佛波酯12-O-十四烷酰佛波醇13-乙酸酯（TPA）（蛋白激酶C的激活剂）的刺激作用不相加。此外，蛋白激酶C的抑制剂1-（异喹啉基磺酰基）-2-甲基哌嗪（H-7）不仅抑制TPA刺激的，也抑制VIP刺激的从¹⁴C-酪氨酸合成¹⁴C-儿茶酚胺。这些结果表明，VIP通过激活酪氨酸羟化酶刺激儿茶酚胺合成，并且蛋白激酶C参与了这种刺激机制。