涤纶人工血管上交联蛋白渗漏产物的高效液相色谱分析

F.p.l.c. analysis of the leakage products of proteins crosslinked on Dacron vascular prostheses.

作者信息

Warocquier-Clérout R, Sigot-Luizard M F, Legendre J M

出版信息

Biomaterials. 1987 Mar;8(2):118-23. doi: 10.1016/0142-9612(87)90100-1.

Abstract

The haemocompatibility of vascular, Dacron prostheses was improved by coating with albumin and/or collagen crosslinked with glutaraldehyde (GTA) or carbodiimide (CDI). F.p.l.c. (fast protein liquid chromatography) analysis of the products desorbed from polymeric matrices incubated in physiological conditions for periods extended up to 10 d did not detect the monomers or polymers of collagen and albumin but small amounts of degradation products, the molecular weights of which were less than 45,000, thus minimizing an eventual immunogenic response after implantation. However GTA and CDI matrices required extensive washing to neutralize the cytotoxic effect of GTA and achieve the release of CDI from protein complexes.

摘要

通过用白蛋白和/或与戊二醛（GTA）或碳二亚胺（CDI）交联的胶原蛋白进行涂层，可改善血管涤纶人工血管的血液相容性。对在生理条件下孵育长达10天的聚合物基质上解吸的产物进行快速蛋白质液相色谱（F.p.l.c.）分析，未检测到胶原蛋白和白蛋白的单体或聚合物，但检测到少量降解产物，其分子量小于45,000，从而将植入后可能的免疫原性反应降至最低。然而，GTA和CDI基质需要大量洗涤以中和GTA的细胞毒性作用并实现CDI从蛋白质复合物中的释放。