Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Clinical College of Anhui Medical University, Shenzhen, Guangdong, 518036, PR China.
Shantou University Medical College, Guangdong, Shantou, 515041, PR China.
Pathol Res Pract. 2019 Jun;215(6):152391. doi: 10.1016/j.prp.2019.03.019. Epub 2019 Mar 19.
The present study aims to determine the association of microRNA-106b-5p (miR-106b-5p) expression with the clinicopathological features and prognosis of renal cell carcinoma (RCC).
The formalin-fixed paraffin-embedded(FFPE) Tumor tissues were collected from 284 RCC patients. The expression of miR-106b-5p was examined by Reverse Transcription Real-time Quantitative Polymerase Chain Reaction (RT-qPCR), followed by correlation analysis with clinicopathological features and prognosis. Patient survival analysis was determined by the Kaplan-Meier method using the log-rank test was used for patient survival analysis, and the univariate and multivariate analysis was determined by a Cox's regression model.
Kaplan-Meier analysis indicated that patients with high miR-106b-5p expression showed a significantly shorter overall survival, compared with patients with low miR-106b-5p expression (p = 0.001). Univariate and multivariate analysis considered miR-106b-5p expression as an independent risk factor for predicting the prognosis of RCC patients. No significant evident showed that the expression level of miR-106b was related to gender, age, tumor size or tumor stage. Also, the results above were verified by analyzing the data of 506 cases from The Cancer Genome Atlas database (TCGA).
The results pointed out that the high expression of miR-106b-5p serves as an independent factor for predicting the worse prognosis of RCC patients.
本研究旨在探讨 microRNA-106b-5p（miR-106b-5p）的表达与肾细胞癌（RCC）的临床病理特征和预后的关系。
收集 284 例 RCC 患者的福尔马林固定石蜡包埋（FFPE）肿瘤组织。采用逆转录实时定量聚合酶链反应（RT-qPCR）检测 miR-106b-5p 的表达，然后进行相关性分析，以探讨其与临床病理特征和预后的关系。采用 Kaplan-Meier 法进行患者生存分析，Log-rank 检验进行单因素和多因素分析，Cox 回归模型进行多因素分析。
Kaplan-Meier 分析表明，miR-106b-5p 高表达组患者的总生存率明显低于 miR-106b-5p 低表达组（p=0.001）。单因素和多因素分析均表明 miR-106b-5p 表达是预测 RCC 患者预后的独立危险因素。miR-106b-5p 的表达水平与性别、年龄、肿瘤大小或肿瘤分期无显著相关性。上述结果在分析 TCGA 数据库的 506 例数据时得到了验证。
miR-106b-5p 的高表达可作为预测 RCC 患者预后不良的独立因素。
