Enzyme and Microbial Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology Delhi, New Delhi 110016, India.
Enzyme and Microbial Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology Delhi, New Delhi 110016, India.
Bioresour Technol. 2019 Sep;287:121391. doi: 10.1016/j.biortech.2019.121391. Epub 2019 May 2.
This work studied the production of Transglutaminase (TGase) using wheat bran as carbon source. The medium components and culture conditions were optimized by statistical Box-Behnken response surface methodology. The release of active Transglutaminase was enhanced by adding (i) protease to remove pro-region to make inactive enzyme to active form, (ii) Cetyl trimethyl ammonium bromide (CTAB) which facilitated more secretion. Under finally optimized conditions viz. 5 g wheat bran, protease: 39.14 U, magnesium chloride (MgCl): 0.10 M, CTAB: 0.08% and inoculation size: 2% led to 4-fold (12.949 ± 0.061 IU/g) increased TGase production over that of un-optimized conditions. The application of TGase was shown to be useful in effective casein cross-linking.
本研究以麦麸作为碳源生产转谷氨酰胺酶（TGase）。通过统计 Box-Behnken 响应面法优化了培养基成分和培养条件。通过添加（i）蛋白酶去除前导肽以使无活性的酶转化为有活性的形式，以及（ii）十六烷基三甲基溴化铵（CTAB）促进更多分泌，来增强活性 TGase 的释放。在最终优化的条件下（即 5g 麦麸、蛋白酶：39.14U、氯化镁（MgCl）：0.10M、CTAB：0.08%和接种量：2%），与未优化条件相比，TGase 的产量增加了 4 倍（12.949±0.061IU/g）。研究表明，TGase 的应用在有效的酪蛋白交联中是有用的。
