Li Zhong-Bo, Fu Yi-Tian, Cheng Tian-Yin, Yao Guo-Min, Hou Qiang-Hong, Li Fen, Zhao Yu, Zou Feng-Cai, Liu Guo-Hua
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan Province, People's Republic of China.
College of Animal Science and Technology, HuaiHua Vocational and Technical College, Huaihua, 418000, Hunan Province, People's Republic of China.
Acta Parasitol. 2019 Jun;64(2):360-366. doi: 10.2478/s11686-019-00053-9. Epub 2019 May 10.
Haemaphysalis longicornis is an important ectoparasite of domestic and wild animals that can transmit many pathogens including viruses, fungi, bacteria and protozoa.
In this study, we examined genetic variation and population genetics in three mitochondrial (mt) genes [cox1 (cytochrome c subunit 1), rrnL (large subunit ribosomal RNA) and nad5 (NADH dehydrogenase 5)] among four H. longicornis populations from China.
The sizes of the partial sequences of cox1, rrnL and nad5 were 776 bp, 409 bp, 510 bp, respectively. Among the obtained sequences, we identified 22 haplotypes for cox1, 2 haplotypes for rrnL and 17 haplotypes for nad5. Low gene flow and significant genetic differentiation (66.2%) were detected among H. longicornis populations. There was no rapid expansion event in the demographic history of four H. longicornis populations in China. In addition, phylogenetic analyses confirmed that all the Haemaphysalis isolates were H. longicornis which were segregated into two major clades.
The mt DNA genes provide a potential novel genetic marker for molecular epidemiology of H. longicornis and assist in the control of tick and tick-borne diseases in humans and animals.
长角血蜱是家畜和野生动物的一种重要外寄生虫，可传播包括病毒、真菌、细菌和原生动物在内的多种病原体。
在本研究中，我们检测了来自中国的四个长角血蜱种群中三个线粒体（mt）基因[细胞色素c亚基1（cox1）、核糖体大亚基RNA（rrnL）和NADH脱氢酶5（nad5）]的遗传变异和群体遗传学。
cox1、rrnL和nad5部分序列的大小分别为776bp、409bp、510bp。在获得的序列中，我们鉴定出cox1的22个单倍型、rrnL的2个单倍型和nad5的17个单倍型。长角血蜱种群间检测到低基因流和显著的遗传分化（66.2%）。中国四个长角血蜱种群的种群历史中没有快速扩张事件。此外，系统发育分析证实所有血蜱分离株均为长角血蜱，分为两个主要分支。
线粒体DNA基因可为长角血蜱的分子流行病学提供一种潜在的新型遗传标记，并有助于控制人和动物的蜱及蜱传疾病。
