Fujian Provincial Key Laboratory for the Prevention & Control of Animal Infectious Diseases &Biotechnology, College of Life Sciences, Longyan University, Longyan, Fujian Province, China.
Engineering Research Center for the Prevention and Control of Animal Original Zoonosis, Fujian Province, College of Life Science, Longyan University, Longyan, Fujian Province, China.
Folia Parasitol (Praha). 2022 Sep 28;69:2022.019. doi: 10.14411/fp.2022.019.
A three-year-old male South China tiger died in the tiger enclosure of the China Tiger Park in the Meihua Mountains on December 2018 after being bitten by a tick. This tiger presented clinical symptoms like whole-body severe jaundice, hepatosplenomegaly, kidney, and lymph node hemorrhages. The Colpodella sp.-specific 18S rRNA gene was detected using nested PCR. Interestingly, the DNA isolated from the blood of the tiger was found to be 100% similar to that of the tick by NCBI BLAST analysis. However, the DNA fragments isolated from the tiger's blood were 90.1% similar to the Colpodella sp. strain human erythrocyte parasite (HEP, MH208621) and 90.4% similar to the Colpodella sp. strain Heilongjiang (HLJ, KT364261). To investigate the species of ticks and ticks-carried Colpodella parasites in this region, the species of ticks obtained from the grasses outside the tiger enclosure and the species of Colpodella carried by ticks were identified. The DNA from ticks as well as that from the tick-borne Colpodella sp. were amplified from each tick using PCR followed by amplicon sequencing. In total 402 adult ticks samples were collected, among which 22 were positive for Colpodella sp. (5.5%), and the species were further determined by morphology, DNA sequencing and phylogenetic analyses. Interestingly, one Colpodella sp. was found to have 94.2% sequence similarities to the Colpodella sp. strain HEP (MH208621). This strain was previously reported to infect a woman in Yunnan, China. In addition, three Colpodella sp. showed 87-91% sequence similarities to the Colpodella sp. strain HLJ (KT364261), which was previously reported to infect human in Heilongjiang, China. This study disclosed the possibility of zoonotic transmission of Colpodella sp. by ticks in China. Finally, it provides a basis for urgently determining and monitoring the repertoire of ticks-borne piroplasmid pathogens, with the ultimate aim of strategic control.
2018 年 12 月，一只三岁雄性华南虎在中国梅花山华南虎公园的虎舍中被蜱虫叮咬后死亡。这只老虎出现了全身严重黄疸、肝脾肿大、肾脏和淋巴结出血等临床症状。使用巢式 PCR 检测到 Colpodella sp.-特异性 18S rRNA 基因。有趣的是，通过 NCBI BLAST 分析发现，从老虎血液中分离出的 DNA 与蜱虫的 DNA 完全相同。然而，从老虎血液中分离出的 DNA 片段与 Colpodella sp. 人红细胞寄生虫（HEP，MH208621）的相似度为 90.1%，与 Colpodella sp. 黑龙江株（HLJ，KT364261）的相似度为 90.4%。为了调查该地区蜱虫和蜱虫携带的 Colpodella 寄生虫的种类，从虎舍外的草丛中采集了蜱虫，并鉴定了蜱虫携带的 Colpodella 种类。使用 PCR 从每只蜱虫中扩增出蜱虫和蜱虫携带的 Colpodella sp. 的 DNA，然后对扩增子进行测序。共采集了 402 只成年蜱虫样本，其中 22 只为 Colpodella sp.（5.5%）阳性，通过形态学、DNA 测序和系统发育分析进一步确定了种类。有趣的是，发现一种 Colpodella sp. 与 Colpodella sp. HEP 株（MH208621）的序列相似度为 94.2%。该菌株曾在中国云南报告感染一名妇女。此外，三种 Colpodella sp. 与 Colpodella sp. HLJ 株（KT364261）的序列相似度为 87-91%，该菌株曾在中国黑龙江报告感染人类。本研究揭示了中国蜱虫传播 Colpodella sp. 的人畜共患传播的可能性。最后，为紧急确定和监测蜱传梨形虫病原体库提供了依据，最终目标是进行战略控制。
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