溴化三乙锡对大鼠和兔脑亚细胞组分中蛋白质磷酸化的影响。

Effects of triethyltin bromide on protein phosphorylation in subcellular fractions from rat and rabbit brain.

作者信息

Neumann P E, Taketa F

出版信息

Brain Res. 1987 Apr;388(1):83-7. doi: 10.1016/0169-328x(87)90024-6.

Abstract

Phosphorylation of specific proteins in subcellular fractions of rat brain is affected by the presence of low concentrations (1-50 microM) of triethyltin bromide (Et3SnBr), in vitro. SDS-PAGE and autoradiography showed that Et3SnBr increased phosphorylation of an Mr = 42,000 phosphorylation of an Mr = 52,000 component. The Mr = 42,000 and 76,000-80,000 phosphoproteins have been identified as the alpha-subunit of pyruvate dehydrogenase (PDH) and synapsin, respectively. Et3SnBr-induced phosphorylation of rabbit brain PDH results in partial inactivation of the PDH complex.

摘要

体外实验中，低浓度（1 - 50微摩尔）的溴化三乙锡（Et3SnBr）会影响大鼠脑亚细胞组分中特定蛋白质的磷酸化。SDS - 聚丙烯酰胺凝胶电泳（SDS - PAGE）和放射自显影显示，Et3SnBr增加了分子量为42,000和52,000组分的磷酸化。分子量为42,000和76,000 - 80,000的磷蛋白分别被鉴定为丙酮酸脱氢酶（PDH）的α亚基和突触素。Et3SnBr诱导兔脑PDH的磷酸化会导致PDH复合物部分失活。