从两名患有肌萎缩侧索硬化症的巴西患者和两名健康受试者中生成四条患者特异性多能诱导干细胞系。
Generation of four patient-specific pluripotent induced stem cell lines from two Brazilian patients with amyotrophic lateral sclerosis and two healthy subjects.
作者信息
Gubert Fernanda, Vasques Juliana F, Cozendey Tatiane D, Domizi Pablo, Toledo María Fernanda, Kasai-Brunswick Tais H, Loureiro Marli P S, Lima José M B, Gress Claudio H, Cabello Giselda M K, Cabello Pedro H, Borgonovo Tamara, Vaz Isadora M, Silva Rosane, Mendez-Otero Rosalia
机构信息
Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil; Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, Brazil; Instituto de Ciências Biomédicas, Rio de Janeiro, Brazil.
Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil; Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, Brazil.
出版信息
Stem Cell Res. 2019 May;37:101448. doi: 10.1016/j.scr.2019.101448. Epub 2019 May 4.
Induced pluripotent stem cell (iPSC) lines were generated from erythroblasts of two patients with amyotrophic lateral sclerosis (ALS) and two healthy individuals. One familial and one sporadic ALS patients were used, both with genetic alterations in VAPB gene. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing the reprogramming factors OCT3/4, KLF4, SOX2 and cMYC) was used to generate the iPSC cell lines. The four iPSCs express pluripotency markers, have normal karyotype and differentiated spontaneously in the three germ layers. The expression of Sendai virus was lost in all iPSC lines after 15 passages.
诱导多能干细胞（iPSC）系由两名肌萎缩侧索硬化症（ALS）患者和两名健康个体的成红细胞生成。使用了一名家族性和一名散发性ALS患者，二者VAPB基因均有基因改变。使用CytoTune™-iPS 2.0仙台重编程试剂盒（包含重编程因子OCT3/4、KLF4、SOX2和cMYC）生成iPSC细胞系。这四个iPSC表达多能性标志物，具有正常核型并能在三个胚层中自发分化。15代后，所有iPSC系中仙台病毒的表达均消失。
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