人膀胱移行细胞癌的体外药敏研究。

In vitro drug sensitivity studies in human transitional cell carcinoma of the bladder.

作者信息

Konowalchuk T W, Elhilali M, Mackillop W J

出版信息

Cancer Lett. 1987 May;35(2):181-90. doi: 10.1016/0304-3835(87)90043-7.

Abstract

An in vitro clonogenic assay was used to study the activity of 3 established cytotoxic drugs and 3 experimental agents against a series of 33 human transitional cell carcinomas. Strict adherence to rigid colony size criteria gave very reproducible results with the 3 cytotoxic drugs (adriamycin, mitomycin C and thiotepa) and our sensitivity curves are of the form expected on the basis of past experience with tumour cell lines. The unexplained plateau form curves which have previously led to criticism of the assay were not seen. Resistant subpopulations were found only in poorly differentiated tumours. This modified assay was then used to test 3 experimental agents for activity against transitional cell carcinoma. Sulphopentosan, DMSO and retinoic acid all proved inactive in this system.

摘要

采用体外克隆形成试验研究3种已确立的细胞毒性药物和3种实验药物对33例人移行细胞癌的活性。严格遵循严格的集落大小标准，3种细胞毒性药物（阿霉素、丝裂霉素C和噻替派）得到了非常可重复的结果，我们的敏感性曲线呈基于以往肿瘤细胞系经验所预期的形式。未见到先前导致对该试验提出批评的无法解释的平台形曲线。仅在低分化肿瘤中发现耐药亚群。然后使用这种改良试验来测试3种实验药物对移行细胞癌的活性。硫酸戊聚糖、二甲基亚砜和视黄酸在该系统中均被证明无活性。