Predoctoral student, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
Resident, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
J Prosthet Dent. 2019 Jun;121(6):966.e1-966.e6. doi: 10.1016/j.prosdent.2019.02.019. Epub 2019 May 8.
Soaking dentures in vinegar or hydrogen peroxide does not seem to remove the microorganisms involved with prosthetic stomatitis efficiently. A mixture of these 2 substances may be effective, but studies are lacking.
The purpose of this in vitro study was to evaluate the antimicrobial effect and cytotoxic activity of vinegar-hydrogen peroxide mixtures against Candida albicans and Staphylococcus aureus.
For antimicrobial tests, planktonic cells and biofilms of C. albicans and S. aureus cultured on acrylic resin disks were exposed to 0.5% sodium hypochlorite; 0.2% peracetic acid; vinegar-hydrogen peroxide mixtures at concentration ratios 1:1, 1:3, and 3:1; vinegar-water mixtures at concentration ratios 1:1, 1:3, and 3:1; and hydrogen peroxide-water mixtures at concentration ratios 1:1, 1:3, and 3:1. Antimicrobial activity was evaluated by counting viable colony-forming units after disinfection. For cytotoxicity tests, the 1:1 vinegar-hydrogen peroxide mixture was serially diluted (10 to 10) and allowed to be in direct contact with HaCaT keratinocytes for 24 hours. Cytotoxicity was quantitatively and qualitatively determined by counting the number of viable cells and analyzing morphological cell changes.
All vinegar-hydrogen peroxide mixtures, sodium hypochlorite, and peracetic acid efficiently eliminated C. albicans and S. aureus (P<.05), whereas vinegar and hydrogen peroxide solutions used separately were not as efficient as the experimental mixtures. The 10 and 10 dilutions of vinegar-hydrogen peroxide solutions were considered noncytotoxic, whereas dilutions below 10 were strongly cytotoxic, comparable with the 10 dilution of 0.2% peracetic acid.
The vinegar-hydrogen peroxide mixture effectively eliminated C. albicans and S. aureus from acrylic resin. Dilutions equal or below 10 of this mixture presented strong cytotoxic effects.
将义齿浸泡在醋或过氧化氢中似乎不能有效地清除与义齿性口炎相关的微生物。这两种物质的混合物可能有效，但缺乏相关研究。
本体外研究旨在评估醋-过氧化氢混合物对白色念珠菌和金黄色葡萄球菌的抗菌效果和细胞毒性作用。
对于抗菌试验，将培养在丙烯酸树脂盘上的白色念珠菌和金黄色葡萄球菌浮游细胞和生物膜暴露于 0.5%次氯酸钠、0.2%过氧乙酸、浓度比为 1:1、1:3 和 3:1 的醋-过氧化氢混合物、浓度比为 1:1、1:3 和 3:1 的醋-水混合物以及浓度比为 1:1、1:3 和 3:1 的过氧化氢-水混合物中。消毒后通过计数活菌形成单位评估抗菌活性。对于细胞毒性试验，将 1:1 的醋-过氧化氢混合物连续稀释（10 至 10），并使其与 HaCaT 角质形成细胞直接接触 24 小时。通过计数活细胞数量和分析细胞形态变化来定量和定性地确定细胞毒性。
所有醋-过氧化氢混合物、次氯酸钠和过氧乙酸均能有效清除白色念珠菌和金黄色葡萄球菌（P<.05），而单独使用醋和过氧化氢溶液则不如实验混合物有效。醋-过氧化氢溶液的 10 和 10 稀释液被认为是非细胞毒性的，而低于 10 的稀释液则具有强烈的细胞毒性，与 0.2%过氧乙酸的 10 稀释液相当。
醋-过氧化氢混合物能有效清除丙烯酸树脂上的白色念珠菌和金黄色葡萄球菌。该混合物的稀释度等于或低于 10 时具有强烈的细胞毒性作用。
