Effects of freeze-thaw cycles on the physicochemical characteristics of animal manure and its phosphorus forms.

The variations of phosphorus (P) in animal manure during freeze-thaw cycles (FTCs) profoundly influence on non-point source P loss in winter. Therefore, understanding how FTCs influence the physicochemical properties of animal manure and its P availability is crucial. In this study, the freeze-thaw treatment was performed by incubating the pig manure at -20 °C for 12 h and at 18 °C for 12 h. The freeze-only treatment was maintained at -20 °C as a control. In addition, the pig manure was kept at two moisture levels during the FTCs and sampled every five cycles. Six forms of P in the manure were extracted and analyzed. After 30 cycles, the dissolved organic carbon had increased from 10.49 to 13.56 g/kg, and the pH had decreased from 6.25 to 5.77. The particles originally >1000 μm were broken into particles <250 μm. The forms of P in manure shifted from Ca-P, occluded P, and residual P towards NHCl-P, Al-P and Fe-P, resulting in a 23% increase in bioavailable P. These variations were highly coincident with the increase in moisture content and FTC frequency. The proportion of particles <38 μm increased by more than 2% after the FTCs, and the manure P was mainly concentrated in these particles, which might be readily washed away by the melt water. Overall, the study indicated that FTCs could enhance the bioavailability of P in pig manure and the mobility of particle-associated P. These findings are significant for reducing animal manure pollution in freeze-thaw season.