School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National University, University, Daehak-ro 80, Buk-ku, Daegu, 41566, Republic of Korea; KNU Institute for Microorganisms, Kyungpook National University, Daehak-ro 80, Buk-ku, Daegu, 41566, Republic of Korea.
School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National University, University, Daehak-ro 80, Buk-ku, Daegu, 41566, Republic of Korea.
Biochem Biophys Res Commun. 2019 Jun 30;514(3):765-771. doi: 10.1016/j.bbrc.2019.05.030. Epub 2019 May 9.
Sphingobium sp. strain SYK-6, an aerobic gram-negative bacillus found in soil, is known for utilizing lignin-derived monoaryls and biaryls as carbon sources and degrading aromatic compounds. The Sphingobium sp. strain SYK-6 genome contains three genes involved in salicylate catabolism: SLG_11260, SLG_11270, and SLG_11280. Here, we report that the gene product of SLG_11280 functions as a maleylpyruvate hydrolase (SsMPH) with K and K values of 166.2 μM and 3.76 min, respectively. This study also reveals the crystal structures of both the apo and pyruvate-manganese ion-bound SsMPH, which revealed that like other fumarylacetoacetate hydrolases, SsMPH dimerizes and has nine unique 3-helices. Molecular docking studies of maleylpyruvate also revealed the likely binding mode of SsMPH and its substrate.
土壤中发现的好氧革兰氏阴性杆菌鞘氨醇单胞菌（Sphingobium sp.）菌株 SYK-6 以利用木质素衍生的单芳基和双芳基作为碳源以及降解芳香族化合物而闻名。鞘氨醇单胞菌（Sphingobium sp.）菌株 SYK-6 基因组包含三个参与水杨酸代谢的基因：SLG_11260、SLG_11270 和 SLG_11280。在这里，我们报告 SLG_11280 的基因产物作为顺丁烯二酸单酰基辅酶 A 水解酶（SsMPH）发挥作用，其 K 和 K 值分别为 166.2μM 和 3.76 min。本研究还揭示了 apo 和丙酮酸-锰离子结合的 SsMPH 的晶体结构，这表明与其他富马酰乙酰乙酸水解酶一样，SsMPH 二聚化并具有 9 个独特的 3-螺旋。顺丁烯二酸单酰基辅酶 A 的分子对接研究还揭示了 SsMPH 及其底物的可能结合模式。
