Rab7 通过抑制 p38MAPK 通路延缓椎间盘退变。
Rab7 delays intervertebral disc degeneration through the inhibition of the p38MAPK pathway.
机构信息
Department of Orthopedics, Shanxi Dayi Hospital Affiliated to Shanxi Medical University, Taiyuan, 030000, China.
Department of Orthopedics, Shanxi Provincial People's Hospital, Taiyuan, 030000, China.
出版信息
Biochem Biophys Res Commun. 2019 Jun 30;514(3):835-841. doi: 10.1016/j.bbrc.2019.04.184. Epub 2019 May 9.
The objective of this study is to determine whether Rab7 could delay intervertebral disc degeneration and study the mechanism. We chose old mice to established intervertebral disc degeneration model and study the effect of Rab7 on intervertebral disc by western blotting, quantitative real-time PCR, immunohistochemistry et al. Nucleus pulposus cells were cultured to study the exact mechanism. Expression of Rab7 was decreased in degenerative human nucleus pulposus tissues. With the aging of mice, the degree of intervertebral disc degeneration was aggravated and the expression of Rab7 was decreased. In addition, overexpression of Rab7 can reduce the degeneration of nucleus pulposus cells and reduce the levels of p38 and p-p38. Rab7 is a protective factor for intervertebral discs and could delays intervertebral disc degeneration through the inhibition of the p38MAPK pathway.
本研究旨在探讨 Rab7 是否可以延缓椎间盘退变，并研究其作用机制。我们选择老年小鼠建立椎间盘退变模型，通过 Western blot、定量实时 PCR、免疫组织化学等方法研究 Rab7 对椎间盘的作用。同时，我们还培养了髓核细胞来研究确切的作用机制。退变的人髓核组织中 Rab7 的表达降低。随着小鼠年龄的增长，椎间盘退变程度加重，Rab7 的表达降低。此外，Rab7 的过表达可以减少髓核细胞的退变，降低 p38 和 p-p38 的水平。Rab7 是椎间盘的保护因子，通过抑制 p38MAPK 通路可以延缓椎间盘退变。
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