登革病毒诱导的急性病毒性脑炎样疾病的小鼠模型

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease.

作者信息

Shen Ting-Jing, Jhan Ming-Kai, Kao Jo-Chi, Ho Min-Ru, Tsai Tsung-Ting, Tseng Po-Chun, Wang Yung-Ting, Lin Chiou-Feng

机构信息

Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University; Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University.

Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University; Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University; Center of Infectious Disease and Signaling Research, National Cheng Kung University;

出版信息

J Vis Exp. 2019 Apr 28(146). doi: 10.3791/59132.

DOI:10.3791/59132

PMID:31081826

Abstract

Dengue virus (DENV), an arthropod-borne virus transmitted by mosquitoes, may cause the severe disease known as dengue hemorrhagic fever, which is characterized by lethal complications due to plasma leakage, ascites, pleural effusion, respiratory distress, severe bleeding, and organ impairment. A few cases of DENV infection present neurological manifestations; however, studies have not explored DENV-induced neuropathogenesis further. In this study, we present a protocol to use an immunocompetent outbred ICR (Institute of Cancer Research) mouse for investigating the induction of central nervous system (CNS) infection with DENV, followed by the progression of acute viral encephalitis-like disease.

摘要

登革病毒（DENV）是一种由蚊子传播的节肢动物传播病毒，可导致称为登革出血热的严重疾病，其特征是由于血浆渗漏、腹水、胸腔积液、呼吸窘迫、严重出血和器官损害而出现致命并发症。少数登革病毒感染病例会出现神经学表现；然而，研究尚未进一步探讨登革病毒诱导的神经发病机制。在本研究中，我们提出了一种方案，使用具有免疫能力的远交系ICR（癌症研究所）小鼠来研究登革病毒诱导的中枢神经系统（CNS）感染，以及随后急性病毒性脑炎样疾病的进展。