Salup R R, Mathieson B J, Wiltrout R H
J Immunol. 1987 Jun 1;138(11):3635-9.
Lymphokine-activated killer (LAK) activity has been proposed to functionally differ from natural killer (NK) activity largely on the basis of a broader target cell spectrum and different kinetics of response to interleukin 2 (IL 2). Similarly, it has been proposed that the precursor cells for LAK activity are phenotypically distinct from NK cells. In most precursor studies, phenotype comparisons have been made between fresh NK cells and LAK cells which have been generated by 3 to 5 days of culture in IL 2. In the present study, we utilized positive selection with monoclonal antibodies to characterize the surface phenotype of precursor cells which give rise to rIL 2-augmented NK activity within 24 hr and to classically generated LAK activity which appears after 3 to 5 days of culture in rIL 2. The results demonstrated that highly purified (93 to 95%) Lyt-2+ or L3T4+ T lymphocytes were unable to generate appreciable amounts of either augmented NK activity or LAK activity when cultured with rIL 2, whereas the highly purified (98%) Lyt-2-, L3T4-, asialo GM1+ lymphocyte subset gave rise to both augmented NK and LAK activities. These findings demonstrate that both augmented NK and LAK activities can arise from precursors expressing the same phenotype. Overall, the results suggest that NK cells in mouse spleen constitute a major precursor component for the generation of LAK activity from that organ.
有人提出，淋巴因子激活的杀伤细胞（LAK）活性在功能上与自然杀伤细胞（NK）活性不同，这主要基于更广泛的靶细胞谱以及对白细胞介素2（IL-2）的不同反应动力学。同样，有人提出LAK活性的前体细胞在表型上与NK细胞不同。在大多数前体细胞研究中，对新鲜NK细胞和在IL-2中培养3至5天产生的LAK细胞进行了表型比较。在本研究中，我们利用单克隆抗体进行阳性选择，以表征在前24小时内产生重组IL-2增强的NK活性以及在重组IL-2中培养3至5天后出现的经典产生的LAK活性的前体细胞的表面表型。结果表明，高度纯化（93%至95%）的Lyt-2⁺或L3T4⁺T淋巴细胞在与重组IL-2一起培养时无法产生可观量的增强NK活性或LAK活性，而高度纯化（98%）的Lyt-2⁻、L3T4⁻、无唾液酸GM1⁺淋巴细胞亚群则产生增强的NK和LAK活性。这些发现表明，增强的NK和LAK活性都可以来自表达相同表型的前体细胞。总体而言，结果表明小鼠脾脏中的NK细胞构成了该器官产生LAK活性的主要前体成分。
