Velkeniers B, Buydens P, Baldys A, De Boel S, Finné E, Golstein J, Vanhaelst L
Life Sci. 1987 Jun 22;40(25):2415-20. doi: 10.1016/0024-3205(87)90756-9.
In adult male Wistar rats submitted to a standardized noise stress, intravenous TRH induced a prolactin (PRL) secretory response. Prior IV naloxone administration not only lowered plasma PRL levels in those stressed rats but abolished also the stimulatory action of TRH. This effect was further studied by superfusion experiments on enriched PRL cell suspensions (70% lactotrophs) from female adult Wistar rats. Naloxone kept unaffected the basal PRL secretion but lowered significantly that induced by TRH. These experiments suggest a dual effect of naloxone on rat PRL secretion, one exerted on central opioid receptors lowering stress-related increased basal PRL levels, the other inhibiting the TRH-dependent PRL secretion exerted at the lactotroph level itself.
在接受标准化噪声应激的成年雄性Wistar大鼠中，静脉注射促甲状腺激素释放激素（TRH）可诱导催乳素（PRL）分泌反应。预先静脉注射纳洛酮不仅降低了那些应激大鼠的血浆PRL水平，还消除了TRH的刺激作用。通过对成年雌性Wistar大鼠富含PRL细胞悬液（70%为泌乳细胞）进行灌流实验进一步研究了这种效应。纳洛酮对基础PRL分泌没有影响，但显著降低了TRH诱导的PRL分泌。这些实验表明纳洛酮对大鼠PRL分泌有双重作用，一是作用于中枢阿片受体，降低与应激相关的基础PRL水平升高，另一是抑制泌乳细胞水平上TRH依赖的PRL分泌。
