Liu Dong, Keiblinger Katharina M, Leitner Sonja, Wegner Uwe, Zimmermann Michael, Fuchs Stephan, Lassek Christian, Riedel Katharina, Zechmeister-Boltenstern Sophie
Institute of Soil Research, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences Vienna (BOKU), Peter Jordan-Straße 82, 1190 Vienna, Austria.
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Microorganisms. 2019 May 13;7(5):129. doi: 10.3390/microorganisms7050129.
Global climate change is predicted to alter drought-precipitation patterns, which will likely affect soil microbial communities and their functions, ultimately shifting microbially-mediated biogeochemical cycles. The present study aims to investigate the simultaneous variation of microbial community compositions and functions in response to drought and following rewetting events, using a soil metaproteomics approach. For this, an established field experiment located in an Austrian forest with two levels (moderate and severe stress) of precipitation manipulation was evaluated. The results showed that fungi were more strongly influenced by drying and rewetting (DRW) than bacteria, and that there was a drastic shift in the fungal community towards a more Ascomycota-dominated community. In terms of functional responses, a larger number of proteins and a higher functional diversity were observed in both moderate and severe DRW treatments compared to the control. Furthermore, in both DRW treatments a rise in proteins assigned to "translation, ribosomal structure, and biogenesis" and "protein synthesis" suggests a boost in microbial cell growth after rewetting. We also found that the changes within intracellular functions were associated to specific phyla, indicating that responses of microbial communities to DRW primarily shifted microbial functions. Microbial communities seem to respond to different levels of DRW stress by changing their functional potential, which may feed back to biogeochemical cycles.
预计全球气候变化将改变干旱-降水模式，这可能会影响土壤微生物群落及其功能，最终改变微生物介导的生物地球化学循环。本研究旨在采用土壤元蛋白质组学方法，研究微生物群落组成和功能在干旱及随后再湿润事件响应中的同时变化。为此，对奥地利森林中一个既定的田间试验进行了评估，该试验有两个降水调控水平（中度和重度胁迫）。结果表明，真菌比细菌受干燥和再湿润（DRW）的影响更大，并且真菌群落发生了剧烈变化，向以子囊菌门为主的群落转变。在功能响应方面，与对照相比，中度和重度DRW处理中均观察到更多数量的蛋白质和更高的功能多样性。此外，在两种DRW处理中，分配给“翻译、核糖体结构和生物发生”以及“蛋白质合成”的蛋白质增加，表明再湿润后微生物细胞生长增强。我们还发现细胞内功能的变化与特定的门类相关，这表明微生物群落对DRW的响应主要改变了微生物功能。微生物群落似乎通过改变其功能潜力来响应不同水平的DRW胁迫，这可能会反馈到生物地球化学循环中。
