Yao Defu, Liu Yingfu, Chen Xiuli, Lim Teck Kwang, Wang Lili, Aweya Jude Juventus, Zhang Yueling, Lin Qingsong
Department of Biology and Guangdong Provincial Key Laboratory of Marine Biotechnology, Faculty of Science, Shantou University, Shantou, 515063, Guangdong, China.
Department of Biological Sciences, Faculty of Science, National University of Singapore, 14 Science Drive 4, Singapore, 117543, Singapore.
Arch Virol. 2019 Jul;164(7):1889-1895. doi: 10.1007/s00705-019-04264-3. Epub 2019 May 13.
Singapore grouper iridovirus (SGIV) is a lethal grouper virus containing 162 predicted ORFs. Previous proteomic studies led to identification of 73 SGIV structural proteins. Here, SDS-assisted tube-gel digestion and DOC-assisted in-solution digestion coupled with LC-ESI-MS/MS were applied to further profile the SGIV structural proteome. We identified a total of 90 SGIV structural proteins including 24 newly reported proteins. Additionally, several PTMs were identified, including 26 N-terminal acetylated proteins, three phosphorylated proteins, and one myristoylated protein. Importantly, 47 of the proteins that were identified are predicted to contain conserved domains. Our work greatly expands the repertoire of the SGIV structural proteome and provides more insight into the biology of SGIV.
新加坡石斑鱼虹彩病毒（SGIV）是一种致死性石斑鱼病毒，含有162个预测的开放阅读框（ORF）。先前的蛋白质组学研究已鉴定出73种SGIV结构蛋白。在此，采用十二烷基硫酸钠（SDS）辅助的试管凝胶消化法和去氧胆酸钠（DOC）辅助的溶液内消化法，并结合液相色谱-电喷雾串联质谱（LC-ESI-MS/MS），进一步分析SGIV的结构蛋白质组。我们共鉴定出90种SGIV结构蛋白，其中包括24种新报道的蛋白。此外，还鉴定出了几种翻译后修饰（PTM），包括26种N端乙酰化蛋白、3种磷酸化蛋白和1种豆蔻酰化蛋白。重要的是，所鉴定出的蛋白质中有47种预计含有保守结构域。我们的工作极大地扩展了SGIV结构蛋白质组的范围，并为深入了解SGIV的生物学特性提供了更多信息。
