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开发并鉴定了西高止山蛙 Indirana semipalmata 的 10 个多态性微卫星位点,并在相关物种中进行了交叉扩增。

Development and characterization of ten polymorphic microsatellites of the Western Ghats frog Indirana semipalmata and cross amplification in related species.

机构信息

Molecular Ecology Laboratory, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, 695014, India.

出版信息

Mol Biol Rep. 2019 Oct;46(5):5487-5492. doi: 10.1007/s11033-019-04854-z. Epub 2019 May 13.

Abstract

The genus Indirana Laurent, 1986 is composed of 14 species which are endemic to the Western Ghats of India. We isolated and evaluated ten polymorphic microsatellite markers in 32 individuals of Indirana semipalmata. The cross amplification test was successful in two Indirana (I. brachytarsus and I. yadera) and one Walkerana (W. leptodactyla, previously known as I. leptodactyla) species endemic to the Western Ghats. The identified markers will be useful for further studies on the conservation genetics of endemic frog species of Western Ghats.

摘要

该属 Indirana Laurent,1986 由 14 种组成,这些物种是印度西高止山脉的特有种。我们从 32 只 Indirana semipalmata 个体中分离并评估了 10 个多态微卫星标记。交叉扩增测试在西高止山脉特有的两个 Indirana(I. brachytarsus 和 I. yadera)和一个 Walkerana(W. leptodactyla,以前称为 I. leptodactyla)物种中成功。鉴定出的标记将对西高止山脉特有青蛙物种的保护遗传学研究有用。

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