寻常型疥疮瘙痒：胸腺基质淋巴细胞生成素和骨膜蛋白过表达诱导巨噬细胞产生的白细胞介素-31。
Pruritus in ordinary scabies: IL-31 from macrophages induced by overexpression of thymic stromal lymphopoietin and periostin.
机构信息
Department of Dermatology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Department of Dermatology, National Defense Medical College, Saitama, Japan.
出版信息
Allergy. 2019 Sep;74(9):1727-1737. doi: 10.1111/all.13870. Epub 2019 Jun 2.
BACKGROUND
Scabies is a common contagious skin disease caused by an infestation of the skin by Sarcoptes scabiei var. hominis. A hallmark symptom of scabies is severe itch.
METHODS
We sought to determine the generation of a pruritogenic cytokine, interleukin (IL)-31, together with immune profiles in skin lesions of ordinary scabies through immunohistochemical and immunofluorescent studies. To elucidate the pathological mechanisms of IL-31 generation, murine peritoneal macrophages were stimulated with various T helper 2 (Th2) cytokines and proteins ex vivo.
RESULTS
A large number of CCR4(+) Th2 cells, eosinophils, and basophils infiltrated in scabies lesions. Increased generation of IL-31, thymic stromal lymphopoietin (TSLP), and periostin was also observed. A major population of IL-31(+) cells were Arginase-1(+)/CD163(+) M2 macrophages. Murine peritoneal macrophages showed an M2 phenotype and generated IL-31 when stimulated with TSLP and periostin.
CONCLUSION
IL-31 appeared to be largely generated by M2 macrophages in ordinary scabies lesions. This IL-31 induction was mediated by TSLP and periostin.
背景
疥疮是一种由人疥螨引起的常见传染性皮肤病。疥疮的一个标志性症状是剧烈瘙痒。
方法
我们通过免疫组织化学和免疫荧光研究，试图确定普通疥疮皮损中致痒细胞因子白细胞介素（IL）-31 的产生及其免疫特征。为了阐明 IL-31 产生的病理机制，我们使用各种辅助性 T 细胞 2（Th2）细胞因子和蛋白质对小鼠腹腔巨噬细胞进行了体外刺激。
结果
大量 CCR4（+）Th2 细胞、嗜酸性粒细胞和嗜碱性粒细胞浸润在疥疮病变中。还观察到白细胞介素（IL）-31、胸腺基质淋巴细胞生成素（TSLP）和骨膜蛋白的生成增加。大量的 IL-31（+）细胞是精氨酸酶-1（+）/CD163（+）M2 巨噬细胞。当用 TSLP 和骨膜蛋白刺激时，小鼠腹腔巨噬细胞表现出 M2 表型并产生 IL-31。
结论
IL-31 似乎主要由普通疥疮病变中的 M2 巨噬细胞产生。这种 IL-31 的诱导是由 TSLP 和骨膜蛋白介导的。
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