Division of Neurological Sciences, Division of Clinical Veterinary Neurology, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Laenggassstrasse 128, 3012, Bern, Switzerland.
Institute of Clinical Chemistry, Inselspital, Bern University Hospital and University of Bern, INO-F3010, Bern, Switzerland.
BMC Vet Res. 2019 May 14;15(1):148. doi: 10.1186/s12917-019-1878-9.
Hemorrhage in the spinal canal leads to further damage of the spinal cord influencing outcome in dogs with intervertebral disk (IVD) extrusion. The aim of the study was to evaluate blood degradation products and ferritin in the cerebrospinal fluid (CSF) of dogs with thoracolumbar IVD extrusion, and their association to clinical parameters and MRI findings.
In the CSF of dogs with IVD extrusion, both net oxyhemoglobin absorption (NOA) and net bilirubin absorption (NBA) were significantly higher compared to the control groups of dogs with steroid responsive meningitis arteritis (SRMA) and idiopathic epilepsy (IE) (P < 0.001), but NOA compared to the idiopathic epilepsy group contaminated artificially with blood (IEc) was not (P = 0.890). Ferritin concentration was significantly higher in dogs with IVD extrusion compared to dogs with IE (P = 0.034), but not to dogs with SRMA (P = 0.526). There was no association between NOA, NBA or ferritin concentration and severity or duration of clinical signs. In dogs with a higher ferritin concentration the outcome was better (P = 0.018). In dogs with evidence of hemorrhage on MRI, NOA and NBA were significantly higher (P = 0.016, P = 0.009), but not ferritin (P = 0.0628).
Quantification of blood degradation products and ferritin in the CSF of dogs to assess subarachnoidal hemorrhage is feasible; however, larger case numbers are needed to evaluate the relevance of NBA and ferritin as prognostic indicators.
椎管内出血会导致脊髓进一步损伤，从而影响患有椎间盘（IVD）突出的犬的预后。本研究的目的是评估患有胸腰椎 IVD 突出的犬的脑脊液（CSF）中血液降解产物和铁蛋白，并将其与临床参数和 MRI 结果相关联。
与类固醇反应性脑膜炎动脉炎（SRMA）和特发性癫痫（IE）对照组相比，患有 IVD 突出的犬的 CSF 中净氧合血红蛋白吸收（NOA）和净胆红素吸收（NBA）均显著升高（P<0.001），但与人为污染有血液的特发性癫痫组相比（IEc）则不然（P=0.890）。患有 IVD 突出的犬的铁蛋白浓度明显高于患有 IE 的犬（P=0.034），但与患有 SRMA 的犬相比则没有差异（P=0.526）。NOA、NBA 或铁蛋白浓度与临床症状的严重程度或持续时间之间没有关联。铁蛋白浓度较高的犬预后较好（P=0.018）。在 MRI 显示有出血迹象的犬中，NOA 和 NBA 明显升高（P=0.016，P=0.009），但铁蛋白没有升高（P=0.0628）。
评估蛛网膜下腔出血时，CSF 中血液降解产物和铁蛋白的定量是可行的；然而，需要更多的病例数量来评估 NBA 和铁蛋白作为预后指标的相关性。
