糖皮质激素受体 α 和 β 在宫内生长受限（IUGR）的人类胎盘中存在性别特异性调节。
Glucocorticoid receptors α and β are modulated sex specifically in human placentas of intrauterine growth restriction (IUGR).
机构信息
Department of Gynecology and Obstetrics, LMU Munich, Maistraße 11, 80337, Munich, Germany.
Department of Pathology, LMU Munich, Thalkirchner Str. 142, 80337, Munich, Germany.
出版信息
Arch Gynecol Obstet. 2019 Aug;300(2):323-335. doi: 10.1007/s00404-019-05189-7. Epub 2019 May 14.
PURPOSE
The objective of this study was to analyze the expression of the glucocorticoid receptor (GR) subtypes GRα and GRβ in placentas affected by intrauterine growth restriction (IUGR).
METHODS
We analyzed the sex-specific placental expression of GRα and GRβ in 23 IUGR and 40 control placentas using immunohistochemistry and immunofluorescence. The GR gene, also known as nuclear receptor subfamily 3 group C member 1 (NR3C1), mRNA production in trophoblast-like cell line BeWo after stimulation with prednisolone was analyzed using quantitative polymerase chain reaction (qPCR) and on the protein level using western blot analysis.
RESULTS
GR subtypes showed a sex-specific upregulation in placentas from IUGR compared to control placentas. An increased expression of GRα was detectable in female placental tissue, whereas GRβ was increased in males.
CONCLUSION
Our data support previous findings suggesting that the glucocorticoid metabolism plays a role in the pathophysiology of IUGR. Furthermore, the data suggest that the underlying molecular mechanisms differ between male and female cases.
目的
本研究旨在分析糖皮质激素受体（GR）亚型 GRα 和 GRβ在宫内生长受限（IUGR）胎盘组织中的表达。
方法
我们使用免疫组织化学和免疫荧光技术分析了 23 例 IUGR 和 40 例对照胎盘组织中 GRα 和 GRβ的性别特异性胎盘表达。使用定量聚合酶链反应（qPCR）和 Western blot 分析在泼尼松龙刺激后胎生细胞系 BeWo 中的 GR 基因（也称为核受体亚家族 3 组 C 成员 1（NR3C1））mRNA 的产生。
结果
与对照组胎盘相比，IUGR 胎盘中的 GR 亚型表现出性别特异性上调。在女性胎盘组织中可检测到 GRα 的表达增加，而在男性中 GRβ 增加。
结论
我们的数据支持先前的研究结果，表明糖皮质激素代谢在 IUGR 的病理生理学中发挥作用。此外，数据表明，男性和女性病例的潜在分子机制不同。
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