Jellum E, Thorsrud A K
Scand J Clin Lab Invest Suppl. 1986;184:71-6.
High resolution two-dimensional (2D)-electrophoresis has been developed to the stage that it is possible to resolve several thousand proteins in cells and tissue, and over six-hundred proteins are separated in human serum. The 2D-technique has been applied to analyses of serum from patients with multiple myeloma, macroglobulinemia and other gammopathies, and to separate apolipoproteins and study abnormalities and polymorphism of these proteins. Cerebrospinal fluid from patients with various neurological diseases has been studied by 2D-electrophoresis and seems to yield information on multiple sclerosis. The 2D-technique has been applied to normal and malignant cells and biopsies, and offers a possibility to detect disease-related proteins. Protein spots from 2D-gels may be used to raise monoclonal antibodies which subsequently can be used to develop simple clinical chemical tests for disease markers. The 2D-electrophoretic method is, however, not yet suitable as a typical routine analysis in the clinical chemistry laboratory, but is primarily a research tool of considerable potential.
高分辨率二维（2D）电泳技术已发展到能够解析细胞和组织中的数千种蛋白质，在人血清中可分离出六百多种蛋白质的阶段。二维技术已应用于多发性骨髓瘤、巨球蛋白血症和其他丙种球蛋白病患者血清的分析，用于分离载脂蛋白并研究这些蛋白质的异常情况和多态性。二维电泳已用于研究各种神经系统疾病患者的脑脊液，似乎能提供有关多发性硬化症的信息。二维技术已应用于正常细胞和恶性细胞以及活检组织，为检测疾病相关蛋白质提供了可能。二维凝胶上的蛋白质斑点可用于制备单克隆抗体，随后可用于开发针对疾病标志物的简单临床化学检测方法。然而，二维电泳方法目前还不适用于临床化学实验室的典型常规分析，主要是一种具有巨大潜力的研究工具。
