Tian Shuang-Shuang, Liu Xiao-Qian, Feng Wei-Hong, Zhang Qi-Wei, Yan Li-Hua, Wang Zhi-Min, Gao Lu
Henan University of Chinese Medicine Zhengzhou 450000, China National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2019 Apr;44(7):1371-1380. doi: 10.19540/j.cnki.cjcmm.20181220.005.
HPLC specific chromatograms of Poria were established, and the concentrations of 10 triterpenoids(16α-hydroxydehydrotrametenolic acid, poricoic acid B, dehydrotumulosic acid, poricoic acid A, polyporenic acid C, poricoic acid AM, 3-O-acetyl-16α-hydroxydehydrotrametenolic acid, dehydropachymic acid, pachymic acid, and dehydrotrametenolic acid) were simultaneously determined. Chromatographic analysis was conducted on a Welch Ultimate XB C_(18) column(4.6 mm × 250 mm,5 μm). Acetonitrile solution(contain 3% tetrahydrofuran)(A) and 0.1% formic acid aqueous solution(B) were used as the mobile phase with gradient elution at a flow rate of 1.0 mL·min~(-1). The column temperature was 30 ℃ and the injection volume was 20 μL. The experimental data were analyzed by the SPSS 22.0 and GraphPad Prism 7.0. The established triterpenoids fingerprints were specific, and the 10 components were well separated and showed good linearity(r≥0.999 6) within the concentration ranges tested. The mean recoveries were between 98.53%-103.8%(RSD 1.7%-2.7%). The method was specific and repeatable, and could be used for identification and quality evaluation of Poria. The results showed that the contents of 10 triterpenoids were positively correlated with each other. The contents of 10 triterpenoids of samples collected from producing areas were higher than that collected from markets. The total contents of 10 triterpenoids of samples collected from Hubei and Yunnan province were slightly higher than that from Anhui province, but the contents of samples from Anhui province were varied in smaller ranges.
建立了茯苓的高效液相色谱（HPLC）特征图谱，同时测定了10种三萜类化合物（16α-羟基去氢栓菌酸、茯苓酸B、去氢土莫酸、茯苓酸A、多孔菌酸C、茯苓酸AM、3 - O - 乙酰基 - 16α - 羟基去氢栓菌酸、去氢茯苓酸、茯苓酸和去氢栓菌酸）的含量。色谱分析采用Welch Ultimate XB C₁₈柱（4.6 mm×250 mm，5μm）。以乙腈溶液（含3%四氢呋喃）（A）和0.1%甲酸水溶液（B）为流动相，进行梯度洗脱，流速为1.0 mL·min⁻¹。柱温为30℃，进样量为20μL。实验数据采用SPSS 22.0和GraphPad Prism 7.0进行分析。所建立的三萜类化合物指纹图谱具有特异性，10种成分分离良好，在所测浓度范围内线性关系良好（r≥0.999 6）。平均回收率在98.53% - 103.8%之间（相对标准偏差1.7% - 2.7%）。该方法具有特异性和重复性，可用于茯苓的鉴别和质量评价。结果表明，10种三萜类化合物的含量之间呈正相关。产地采集的样品中10种三萜类化合物的含量高于市场采集样品。湖北和云南采集的样品中10种三萜类化合物的总含量略高于安徽采集的样品，但安徽样品的含量范围变化较小。
