酶联免疫吸附测定法检测结核性脑膜炎患者脑脊液中的分枝杆菌抗原
Enzyme-linked immunosorbent assay for the detection of mycobacterial antigens in the cerebrospinal fluid in tuberculous meningitis.
作者信息
Donald P R, Cooper R C
出版信息
S Afr Med J. 1987 Jun 6;71(11):699-700.
PMID:3109040
Abstract
Fifty-three cerebrospinal fluid specimens from meningitis patients were investigated by enzyme-linked immunosorbent assay for detection of mycobacterial antigens. After heating at 56 degrees C for 1 hour to eliminate nonspecific interference, all 22 tuberculous meningitis (TBM) specimens had an optical density of greater than 0.05 (sensitivity 100%). Six out of 31 non-TBM cases gave false-positive results (specificity 81%).
摘要
采用酶联免疫吸附测定法对53份脑膜炎患者的脑脊液标本进行检测，以检测分枝杆菌抗原。在56℃加热1小时以消除非特异性干扰后，所有22份结核性脑膜炎（TBM）标本的光密度均大于0.05（敏感性100%）。31例非TBM病例中有6例出现假阳性结果（特异性81%）。
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