Skjønsberg O H, Gravem K, Kierulf P, Godal H C
Thromb Res. 1987 Mar 1;45(5):625-34. doi: 10.1016/0049-3848(87)90325-2.
In order to evaluate the influence of heat treatment (68 degrees C for 24 or 72 hours) on the essential components of antihaemophilic cryoprecipitate, i.e. factor VIII coagulant activity (VIII:C), von Willebrand factor (VIIIR:Ag and VIIIR:RCF) and fibrinogen, ordinary lyophilized cryoprecipitate was compared to heat treated, aminoacid-enriched specimens. The median reduction in factors VIII:C, VIIIR:Ag, VIIIR:RCF and fibrinogen during lyophilization of ordinary cryoprecipitate was 26 per cent, 11 per cent, 1 per cent and 8.5 per cent, respectively. Heat treatment of such cryoprecipitate resulted in 85 to 98.5 per cent reduction in these parameters, while the reduction following lyophilization and heat treatment (24 hours) of aminoacid-containing preparations was not significantly different from non-heated, ordinary cryoprecipitate. Following heating of aminoacid-enriched cryoprecipitate for 72 hours, only factor VIIIR:RCF was significantly reduced (32.5 per cent) compared to non-heated samples. Ordinary cryoprecipitate was almost insoluble following heat treatment. Enrichment with aminoacids, however, made the heat treated cryoprecipitate fully soluble, but the content of these vials were slightly slower in dissolving than non-heated preparations. Ultracentrifugation prior to lyophilization and heating did not improve the solubility. If heat treatment proves to be efficient in inactivating viral agents, we conclude that heated (68 degrees C for 24 hours), aminoacid-enriched cryoprecipitate may be a convenient product for treating haemophilia A, von Willebrand's disease and hypofibrinogenemia.
为了评估热处理（68摄氏度，24或72小时）对抗血友病冷沉淀的主要成分，即因子VIII凝血活性（VIII:C）、血管性血友病因子（VIIIR:Ag和VIIIR:RCF）和纤维蛋白原的影响，将普通冻干冷沉淀与热处理的富含氨基酸的样本进行了比较。普通冷沉淀冻干过程中因子VIII:C、VIIIR:Ag、VIIIR:RCF和纤维蛋白原的中位数减少分别为26%、11%、1%和8.5%。对这种冷沉淀进行热处理导致这些参数减少85%至98.5%，而含氨基酸制剂冻干和热处理（24小时）后的减少与未加热的普通冷沉淀无显著差异。富含氨基酸的冷沉淀加热72小时后，与未加热样本相比，只有因子VIIIR:RCF显著减少（32.5%）。普通冷沉淀热处理后几乎不溶。然而，用氨基酸富集使热处理后的冷沉淀完全可溶，但这些小瓶中的内容物溶解速度比未加热制剂略慢。冻干和加热前进行超速离心并不能提高溶解度。如果热处理被证明能有效灭活病毒制剂，我们得出结论，加热（68摄氏度，24小时）的富含氨基酸的冷沉淀可能是治疗甲型血友病、血管性血友病和低纤维蛋白原血症的方便产品。
