Feng Jie, Zhong Jiale, Tang Xinjing
State Key Laboratory of Natural and Biomimetic Drugs, The School of Pharmaceutical Sciences, Peking University, No. 38, Xueyuan Rd., Beijing, 100191, China.
Anal Bioanal Chem. 2019 Jun;411(16):3613-3619. doi: 10.1007/s00216-019-01843-w. Epub 2019 May 15.
The cyanate anion (CNO), formed spontaneously within cells from urea and carbamoyl phosphate, usually functions as a biomarker of some diseases such as chronic kidney disease. Therefore, accurate determination of CNO is highly demanded. Herein, a 3-amino-2-naphthoic acid-based "turn-on" fluorescence probe was developed for specific detection of CNO. Upon the addition of sodium cyanate, the weak-fluorescent 3-amino-2-naphthoic acid could react with CNO, which triggered intense emission of green fluorescence. And up to 9-fold fluorescence enhancement was observed. The fluorescence enhancement ratios displayed a good linear relationship with the concentrations of CNO in the range of 0.5-200 μM. The high selectivity and sensitivity for CNO detection were investigated with the detection limit as low as 260 nM. The probe was further successfully applied to determine CNO in real samples such as tap water, human urine and serum samples, which offered a promising approach in practical applications. Graphical abstract.
氰酸根阴离子（CNO）由尿素和氨基甲酰磷酸在细胞内自发形成，通常作为某些疾病如慢性肾病的生物标志物。因此，对CNO的准确测定有很高的要求。在此，开发了一种基于3-氨基-2-萘甲酸的“开启”荧光探针用于特异性检测CNO。加入氰酸钠后，弱荧光的3-氨基-2-萘甲酸能与CNO反应，引发强烈的绿色荧光发射。观察到荧光增强高达9倍。荧光增强率与0.5-200μM范围内的CNO浓度呈现良好的线性关系。研究了该探针检测CNO的高选择性和灵敏度，检测限低至260 nM。该探针进一步成功应用于自来水、人尿液和血清样品等实际样品中CNO的测定，为实际应用提供了一种有前景的方法。图形摘要。
