Takuma T, Takeda K, Konno K
Biochem Biophys Res Commun. 1987 May 29;145(1):514-21. doi: 10.1016/0006-291x(87)91351-9.
Natural or recombinant human tumor necrosis factor (TNF) induced NBT-reducing activity of ML-1 cells in a dose-dependent manner. Interferon-gamma (IFN-gamma) induced NBT-reducing activity only marginally. However, when IFN-gamma was combined with TNF, induction of NBT-reducing activity was remarkably increased. IFN-alpha or -beta had almost no effect on the induction of NBT-reducing activity of ML-1 cells, either alone or in combination with TNF. Treatment with both TNF and IFN-gamma synergistically enhanced morphological changes, growth inhibition and activity of Fc receptors, and NBT reduction in ML-1 cells, but not phagocytic activity. The TNF treated cells were classified as macrophage-like by morphology, and by lineage-specific alpha-naphthyl acetate esterase stain. The results indicate that combinations of TNF and IFN-gamma act synergistically in the induction of differentiation of human myeloblastic ML-1 cells.
天然或重组人肿瘤坏死因子（TNF）以剂量依赖的方式诱导ML-1细胞的NBT还原活性。γ干扰素（IFN-γ）仅轻微诱导NBT还原活性。然而，当IFN-γ与TNF联合使用时，NBT还原活性的诱导显著增加。α或β干扰素单独或与TNF联合使用时，对ML-1细胞NBT还原活性的诱导几乎没有影响。TNF和IFN-γ联合处理可协同增强ML-1细胞的形态变化、生长抑制、Fc受体活性和NBT还原，但不增强吞噬活性。经TNF处理的细胞通过形态学以及谱系特异性α-萘乙酸酯酶染色被归类为巨噬细胞样。结果表明，TNF和IFN-γ组合在诱导人髓母细胞ML-1细胞分化中起协同作用。
