Song T, Liu B, Xu B, Yang C
Institute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai, China.
AccuTarget MediPharma (Shanghai) Co. Ltd, Shanghai, China.
Cryo Letters. 2019 May/Jun;40(3):164-172.
Cryosurgery is effectively used for treating various diseases including cancer and tumor. But there is difficulty accurately quantifying the spatial temperature profile needed to determine diseased tissue destruction.
A novel flexible cryosurgical probe was developed that can pass through the natural cavities of human body to minimize the damage to normal tissues. The present study aims to study the design of the flexible cryoprobe by 3D heat transfer modeling and experimental verification.
A 3D model, on the basis of the flexible cryoprobe system, was developed to predict the temperature distribution during cryosurgery in porcine liver. In vitro experiments and pathological analysis with TUNEL staining of tissue samples were performed for verification.
The results of numerical simulation based on the Pennes equation exhibited good agreement with experimental measurements for the flexible cryoprobe system. The simulation shows that the tissue within the range of 0 to 6 mm along the cryoprobe is effectively destroyed and the temperature in this domain decreases to the range -35°C to -40°C. Due to the non-uniform distribution, the temperature in the z-axis direction decreases to -40°C faster, with more serious tissue damage; whereas the x-axis direction has the greater distance of tissue destruction. Pathologic analysis of tissue after in vitro cryosurgery has verified the efficacy of the flexible cryoprobe system.
Numerical simulation and experimental verification demonstrate the efficacy of the flexible cryoprobe system. The 3D heat transfer model can be a valuable tool for optimizing the design of the flexible cryoprobe system.
冷冻手术被有效地用于治疗包括癌症和肿瘤在内的各种疾病。但是，准确量化确定病变组织破坏所需的空间温度分布存在困难。
开发一种新型柔性冷冻手术探针，其能够穿过人体自然腔道以将对正常组织的损伤降至最低。本研究旨在通过三维热传递建模和实验验证来研究柔性冷冻探针的设计。
基于柔性冷冻探针系统开发了一个三维模型，以预测猪肝冷冻手术过程中的温度分布。进行了体外实验以及对组织样本进行TUNEL染色的病理分析以进行验证。
基于佩恩斯方程的数值模拟结果与柔性冷冻探针系统的实验测量结果显示出良好的一致性。模拟表明，沿着冷冻探针0至6毫米范围内的组织被有效破坏，并且该区域内的温度降至-35°C至-40°C的范围。由于分布不均匀，z轴方向的温度更快降至-40°C，组织损伤更严重；而x轴方向的组织破坏距离更大。体外冷冻手术后组织的病理分析验证了柔性冷冻探针系统的有效性。
数值模拟和实验验证证明了柔性冷冻探针系统的有效性。三维热传递模型可以成为优化柔性冷冻探针系统设计的有价值工具。
