Weinstock J V, Blum A M
Cell Immunol. 1987 Jul;107(2):273-80. doi: 10.1016/0008-8749(87)90236-x.
Components of the angiotensin system are present in granulomas of murine schistosomiasis mansoni. Angiotensins may have immunoregulatory function. Granuloma macrophages cultured for up to 3 days generated substantial angiotensin I (AI) and angiotensin II (AII) which appeared in the culture supernatants. Macrophage monolayers were incubated with 3H-labeled amino acids, and culture supernatants were extracted with acetone and analyzed by HPLC. Radiolabeled products eluted at times corresponding to those of authentic angiotensins. Immunoadsorption of angiotensins with angiotensin antisera removed reputed radiolabeled angiotensins from the supernatants. Treatment of the elution fraction corresponding to that of authentic AI with angiotensin-converting enzyme resulted in the generation of radiolabeled polypeptides which coeluted with authentic AII and His-Leu. Similar experiments conducted with nonadherent granuloma cells devoid of macrophages failed to demonstrate angiotensin production. These results suggest that granuloma macrophages can synthesize angiotensins.
血管紧张素系统的成分存在于曼氏血吸虫病小鼠的肉芽肿中。血管紧张素可能具有免疫调节功能。培养长达3天的肉芽肿巨噬细胞产生了大量的血管紧张素I（AI）和血管紧张素II（AII），它们出现在培养上清液中。巨噬细胞单层与3H标记的氨基酸一起孵育，培养上清液用丙酮提取并通过HPLC分析。放射性标记产物的洗脱时间与 authentic 血管紧张素的洗脱时间相对应。用血管紧张素抗血清对血管紧张素进行免疫吸附可从上清液中去除所谓的放射性标记血管紧张素。用血管紧张素转换酶处理与 authentic AI 相对应的洗脱级分，导致产生与 authentic AII 和 His-Leu 共洗脱的放射性标记多肽。对不含巨噬细胞的非贴壁肉芽肿细胞进行的类似实验未能证明血管紧张素的产生。这些结果表明肉芽肿巨噬细胞可以合成血管紧张素。
