Russell J C
Clin Chem. 1987 Jul;33(7):1177-84.
The mechanism of a new fluorescence polarization method for measuring phospholipid in amniotic fluid is described. The fluorescence polarization of a surfactant fluorescent dye solubilized in amniotic fluid correlates significantly with the L/S ratio. Model studies indicate that this results primarily from partitioning of the dye between endogenous albumin, which causes a high fluorescence polarization, and dispersed phospholipid, which causes a much lower polarization. The fluorescence polarization can be compared with a calibration curve to give the ratio of phospholipid to albumin in the sample. This procedure may prove useful in antenatal assessment of fetal lung maturity.
本文描述了一种用于测量羊水磷脂的新型荧光偏振法的机制。溶解在羊水中的表面活性剂荧光染料的荧光偏振与L/S比值显著相关。模型研究表明，这主要是由于染料在内源性白蛋白（导致高荧光偏振）和分散的磷脂（导致低得多的偏振）之间的分配所致。荧光偏振可与校准曲线进行比较，以得出样品中磷脂与白蛋白的比例。该方法可能在胎儿肺成熟度的产前评估中证明有用。
