• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

环状芽孢杆菌淀粉酶基因的分子克隆

Molecular cloning of an amylase gene of Bacillus circulans.

作者信息

Nishizawa M, Ozawa F, Hishinuma F

出版信息

DNA. 1987 Jun;6(3):255-65. doi: 10.1089/dna.1987.6.255.

DOI:10.1089/dna.1987.6.255
PMID:3109866
Abstract

An amylase gene of Bacillus circulans was cloned in B. subtilis and its nucleotide sequence was determined. The putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. Homologous regions with other amylases of Bacillus species were found. A sigma 55-type promoter is located at about 250 bp upstream from the starting codon. This promoter was also functional in Escherichia coli, and able to express beta-galactosidase activity.

摘要

将环状芽孢杆菌的淀粉酶基因克隆到枯草芽孢杆菌中，并测定了其核苷酸序列。推测的前淀粉酶由528个氨基酸组成，分子量为58,776。发现了与芽孢杆菌属其他淀粉酶的同源区域。一个σ55型启动子位于起始密码子上游约250 bp处。该启动子在大肠杆菌中也有功能，能够表达β-半乳糖苷酶活性。

相似文献

1
Molecular cloning of an amylase gene of Bacillus circulans.环状芽孢杆菌淀粉酶基因的分子克隆
DNA. 1987 Jun;6(3):255-65. doi: 10.1089/dna.1987.6.255.
2
[Cloning of the gene encoding a thermostable alpha-amylase from bacillus licheniformis CICIM B0204 and functional identification of its promoter].[地衣芽孢杆菌CICIM B0204耐热α-淀粉酶编码基因的克隆及其启动子的功能鉴定]
Wei Sheng Wu Xue Bao. 2006 Aug;46(4):576-80.
3
Cloning, sequencing, and expression of the genes encoding an isocyclomaltooligosaccharide glucanotransferase and an alpha-amylase from a Bacillus circulans strain.来自环状芽孢杆菌菌株的一种异麦芽低聚糖葡聚糖转移酶和一种α-淀粉酶编码基因的克隆、测序及表达
Biosci Biotechnol Biochem. 2006 Nov;70(11):2690-702. doi: 10.1271/bbb.60294. Epub 2006 Nov 7.
4
Cloning and expression of raw-starch-digesting alpha-amylase gene from Bacillus circulans F-2 in Escherichia coli.环状芽孢杆菌F-2中可消化生淀粉的α-淀粉酶基因在大肠杆菌中的克隆与表达
Biochim Biophys Acta. 1990 Apr 6;1048(2-3):223-30. doi: 10.1016/0167-4781(90)90060-f.
5
Cloning and sequencing of a gene encoding acidophilic amylase from Bacillus acidocaldarius.嗜酸热硫化叶菌嗜酸淀粉酶编码基因的克隆与测序
J Gen Microbiol. 1993 Oct;139(10):2399-407. doi: 10.1099/00221287-139-10-2399.
6
Use of a new catabolite repression resistant promoter isolated from Bacillus subtilis KCC103 for hyper-production of recombinant enzymes.利用从枯草芽孢杆菌KCC103中分离出的一种新的抗分解代谢物阻遏启动子高效生产重组酶。
Protein Expr Purif. 2010 Mar;70(1):122-8. doi: 10.1016/j.pep.2009.09.020. Epub 2009 Oct 6.
7
Deletion analysis of the C-terminal region of the alpha-amylase of Bacillus sp. strain TS-23.芽孢杆菌属TS-23菌株α-淀粉酶C端区域的缺失分析
Arch Microbiol. 2002 Aug;178(2):115-23. doi: 10.1007/s00203-002-0431-5. Epub 2002 May 8.
8
Highly efficient gene expression of a fibrinolytic enzyme (subtilisin DFE) in Bacillus subtilis mediated by the promoter of alpha-amylase gene from Bacillus amyloliquefaciens.由解淀粉芽孢杆菌α-淀粉酶基因启动子介导的枯草芽孢杆菌中纤溶酶（枯草杆菌蛋白酶DFE）的高效基因表达。
Biotechnol Lett. 2004 Sep;26(17):1365-9. doi: 10.1023/B:BILE.0000045634.46909.2b.
9
The N-terminal signal sequence and the last 98 amino acids are not essential for the secretion of Bacillus sp. TS-23 alpha-amylase in Escherichia coli.N 端信号序列和最后 98 个氨基酸对于芽孢杆菌属 TS-23 α-淀粉酶在大肠杆菌中的分泌并非必不可少。
Curr Microbiol. 2001 Sep;43(3):170-5. doi: 10.1007/s002840010282.
10
Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.枯草芽孢杆菌dnaK基因座的克隆、测序及分子分析。
J Bacteriol. 1992 May;174(10):3300-10. doi: 10.1128/jb.174.10.3300-3310.1992.

引用本文的文献

1
Molecular structure of the SWA2 gene encoding an AMY1-related alpha-amylase from Schwanniomyces occidentalis.编码来自西方许旺酵母的一种与AMY1相关的α-淀粉酶的SWA2基因的分子结构。
Curr Genet. 1993 Jul-Aug;24(1-2):75-83. doi: 10.1007/BF00324668.
2
Starch- and glycogen-debranching and branching enzymes: prediction of structural features of the catalytic (beta/alpha)8-barrel domain and evolutionary relationship to other amylolytic enzymes.淀粉和糖原脱支酶与分支酶：催化性（β/α）8桶结构域的结构特征预测及其与其他淀粉分解酶的进化关系
J Protein Chem. 1993 Dec;12(6):791-805. doi: 10.1007/BF01024938.
3
Characteristic differences in the primary structure allow discrimination of cyclodextrin glucanotransferases from alpha-amylases.
一级结构上的特征差异使得能够区分环糊精葡糖基转移酶和α淀粉酶。
Biochem J. 1995 Jan 15;305 ( Pt 2)(Pt 2):685-6. doi: 10.1042/bj3050685.
4
Evidence for movement of the alpha-amylase gene into two phylogenetically distant Bacillus stearothermophilus strains.α-淀粉酶基因转移至两株系统发育关系较远的嗜热脂肪芽孢杆菌菌株的证据。
J Bacteriol. 1988 Mar;170(3):1034-40. doi: 10.1128/jb.170.3.1034-1040.1988.
5
Expression of a Bacillus alpha-amylase gene in yeast.芽孢杆菌α-淀粉酶基因在酵母中的表达。
Curr Genet. 1988 Jul;14(1):1-8. doi: 10.1007/BF00405846.
6
A super-secondary structure predicted to be common to several alpha-1,4-D-glucan-cleaving enzymes.一种预测为几种α-1,4-D-葡聚糖裂解酶共有的超二级结构。
Biochem J. 1989 Apr 1;259(1):145-52. doi: 10.1042/bj2590145.
7
Cloning, characterization and expression of an alpha-amylase gene from Streptomyces griseus IMRU3570.来自灰色链霉菌IMRU3570的α-淀粉酶基因的克隆、表征及表达
Mol Gen Genet. 1991 Feb;225(2):278-88. doi: 10.1007/BF00269860.
8
Cloning and sequencing of a cyclodextrin glucanotransferase gene from Bacillus ohbensis and its expression in Escherichia coli.
Appl Microbiol Biotechnol. 1991 Aug;35(5):600-5. doi: 10.1007/BF00169623.
9
Highly homologous cyclodextrin glycosyltransferases from Bacillus circulans strain 8 and a strain of Bacillus licheniformis.来自环状芽孢杆菌8株和地衣芽孢杆菌一株的高度同源环糊精糖基转移酶。
Appl Microbiol Biotechnol. 1990 Nov;34(2):229-30. doi: 10.1007/BF00166786.
10
Molecular cloning, nucleotide sequence and expression in Escherichia coli of the beta-cyclodextrin glycosyltransferase gene from Bacillus circulans strain no. 8.环状芽孢杆菌8号菌株β-环糊精糖基转移酶基因的分子克隆、核苷酸序列及在大肠杆菌中的表达
Appl Microbiol Biotechnol. 1990 Aug;33(5):542-6. doi: 10.1007/BF00172548.