Kulhari Hitesh, Jangid Ashok K, Adams David J
School of Nano Sciences, Central University of Gujarat, Gandhinagar, India.
Illawarra Health and Medical Research Institute (IHMRI), University of Wollongong, Wollongong, NSW, Australia.
Methods Mol Biol. 2019;1974:195-201. doi: 10.1007/978-1-4939-9220-1_14.
Small interfering RNA (siRNA) is a promising tool for gene therapy-based disease treatments. However, delivery of siRNA to the target cells requires a specific and reliable carrier system. Herein we describe a targeted carrier system that can deliver siRNA to cancer cells overexpressing the human epidermal growth factor 2 (HER2) receptor. Trastuzumab-conjugated poly(amido)amine dendrimers can be synthesized using the protocols described here.
小干扰RNA（siRNA）是基于基因治疗的疾病治疗中有前景的工具。然而，将siRNA递送至靶细胞需要特定且可靠的载体系统。在此，我们描述了一种靶向载体系统，其能够将siRNA递送至过表达人表皮生长因子2（HER2）受体的癌细胞。使用本文所述的方案可以合成曲妥珠单抗偶联的聚（酰胺）胺树枝状大分子。
