Boda K, Brzobohatý B, Baranov V S, Tkadlecek L, Kovác L
Folia Biol (Praha). 1987;33(2):93-7.
Liposomes were loaded with fluorescence-labelled serum albumin and their interaction with mouse spermatozoa was examined in a fluorescence microscope. In the presence of polyethylene glycol 1500, fluorescence became unevenly distributed along the entire body of mature spermatozoa, mostly in the form of aggregates, indicating adsorption of liposomes to the surface of the cells. The fluorescence could be largely removed by washing the cells. In the case of immature sperm cells, fluorescence was evenly distributed on the cells and was not removed by extensive washing, suggesting an internalization of the labelled albumin. A more direct evidence for the entrance of liposome-entrapped macromolecules into the immature sperm cells was provided by autoradiography: radioactivity was found in discrete spots in spermatocytes upon their incubation with liposomes containing isotopically labelled DNA.
脂质体装载了荧光标记的血清白蛋白，并在荧光显微镜下检测了它们与小鼠精子的相互作用。在聚乙二醇1500存在的情况下，荧光沿着成熟精子的整个身体不均匀分布，主要呈聚集形式，表明脂质体吸附到细胞表面。通过洗涤细胞，荧光可以大部分被去除。对于未成熟的精子细胞，荧光均匀分布在细胞上，并且通过大量洗涤也不会被去除，这表明标记的白蛋白被内化。放射自显影为脂质体包裹的大分子进入未成熟精子细胞提供了更直接的证据：当用含有同位素标记DNA的脂质体孵育时，在精母细胞的离散斑点中发现了放射性。
