Department of Plant Biotechnology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore, Karnataka, India.
J Appl Microbiol. 2019 Aug;127(2):556-564. doi: 10.1111/jam.14328. Epub 2019 Jul 1.
To assess phylogenetic and genotypic diversity of Metarhizium anisopliae lineage within diverse agroecosystems in the Karnataka State of India and to compare their chitinase activity and pathogenicity against insect pest of field crops subterranean termite, Odontotermes obesus.
Three phylogenetic and 27 microsatellite markers were used to assess the genetic diversity of M. anisopliae lineage within multiple agroecosystems. Multilocus phylogeny of the Metarhizium isolates identified two species: Metarhizium pingshaense and Metarhizium guizhouense. Multilocus phylogeny and microsatellite markers resolved two phylogenetic species of M. pingshaense, Mp_1 and Mp_2, and one phylogenetic species of M. guizhouense, Mg_1. Phylogenetic species, Mp_2 and Mg_1, were detected with one genotype each and Mp_1 with eleven genotypes. Metarhizium pingshaense GKVK 02_16 isolate caused significantly high mortality of O. obesus in bioassays and detected with high chitinase activity.
The study revealed phylogenetic and genotypic diversity of M. anisopliae lineage in agroecosystems of Karnataka State. Findings of pathogenicity and chitinase activity suggest that M. pingshaense GKVK 02_16 isolate provides effective control of O. obesus.
The investigation provided an understanding of the genetic diversity and biocontrol efficiency of M. anisopliae lineage in agroecosystem. These data will serve as a resource in the future pest management strategies at a regional scale.
评估印度卡纳塔克邦不同农业生态系统中绿僵菌系的系统发育和基因型多样性，并比较其几丁质酶活性和对地下害虫——黄脊竹蝗的致病性。
使用三种系统发育和 27 个微卫星标记评估了多个农业生态系统中绿僵菌系的遗传多样性。绿僵菌分离株的多基因系统发育确定了两个种：平沙绿僵菌和贵州绿僵菌。多基因系统发育和微卫星标记解析了两个绿僵菌种，Mp_1 和 Mp_2，以及一个贵州绿僵菌种，Mg_1。Mp_2 和 Mg_1 被检测到各有一种基因型，而 Mp_1 则有 11 种基因型。绿僵菌平沙菌株 GKVK 02_16 引起黄脊竹蝗的死亡率显著升高，且检测到高几丁质酶活性。
本研究揭示了卡纳塔克邦农业生态系统中绿僵菌系的系统发育和基因型多样性。致病性和几丁质酶活性的研究结果表明，绿僵菌平沙菌株 GKVK 02_16 为黄脊竹蝗提供了有效的控制。
该研究提供了对农业生态系统中绿僵菌系遗传多样性和生物防治效率的理解。这些数据将为未来在区域范围内制定害虫管理策略提供资源。
