12 株源自先天性震颤新生仔猪的新型非典型猪瘟病毒基因组：一个新出现的分支在中国的高流行率。

12 novel atypical porcine pestivirus genomes from neonatal piglets with congenital tremors: A newly emerging branch and high prevalence in China.

机构信息

College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China.

College of Animal Science, South China Agricultural University, Guangzhou, China.

出版信息

Virology. 2019 Jul;533:50-58. doi: 10.1016/j.virol.2019.04.010. Epub 2019 May 3.

DOI:10.1016/j.virol.2019.04.010

PMID:31103885

Abstract

Atypical porcine pestivirus (APPV), a newly discovered member of the genus Pestivirus, is considered to be associated with congenital tremors (CT) in piglets. From June 2016 to January 2018, 440 serum and tissue samples from CT-affected piglets in Anhui, Guangdong and Guangxi provinces were collected to detect APPV. The results showed a high level of 63.4% preference for APPV in 27 swine farms and complicated co-infection cases between APPV and other 12 swine viruses. Meanwhile, 12 novel APPV genomes were screened and identified. Results showed that complete genomes, N and NS5A genes of these novel 12 APPV sequences revealed 80.5%-99.8%, 78%-100% and 76.9%-99.8% nucleotide identities, respectively. Phylogenetic analyses based on sequences of full-length genomes, N and NS5A genes of APPV indicated three well-defined clades including a newly emerging branch in China. This study provides novel epidemiological information of APPV in China.

摘要

非典型猪瘟病毒（APPV）是新近发现的瘟病毒属成员，被认为与仔猪先天性震颤（CT）有关。2016 年 6 月至 2018 年 1 月，从安徽、广东和广西受 CT 影响的仔猪中采集了 440 份血清和组织样本，以检测 APPV。结果显示，在 27 个猪场中，APPV 的偏好率高达 63.4%，且 APPV 与其他 12 种猪病毒存在复杂的合并感染情况。同时，筛选并鉴定了 12 种新的 APPV 基因组。结果表明，这 12 种新型 APPV 序列的完整基因组、N 基因和 NS5A 基因的核苷酸同一性分别为 80.5%-99.8%、78%-100%和 76.9%-99.8%。基于 APPV 全长基因组、N 基因和 NS5A 基因序列的系统进化分析表明，包括中国新出现的分支在内，存在三个明确的进化枝。本研究为中国 APPV 的流行情况提供了新的流行病学信息。