Tsai S C, Gaffney E V
J Natl Cancer Inst. 1987 Jul;79(1):77-81.
The growth-modulating effects of recombinant alpha- and beta-forms of human interleukin-1 (IL-1) and interferon-gamma (IFN-gamma) were examined with several human cell lines. Exposure to combinations of IL-1 and IFN-gamma resulted in three categories of cell response. The first was cell lines in which IL-1 stimulated growth and offset the growth inhibitory effects of IFN-gamma. These lines included the lung carcinoma CALU-1 and the colon carcinoma SW-48. The second was some of the cell lines that were refractory to IL-1 and that were inhibited by IFN-gamma alone. These included the cervical carcinoma HeLa, the transformed milk line HBL-100, and the myelogenous leukemia K562. The third group consisted of cells in which growth inhibition by IL-1 and IFN-gamma was additive. These included the mammary carcinomas MCF-7 and MDA-MB-415. The exception to this latter group was ME-180 in which significant additive inhibitory effects could not be demonstrated. IL-1 alone primarily induced a cytostatic effect in growth-inhibited cell lines. The cytolytic effect induced by IFN-gamma was increased in the presence of IL-1. The data support the conclusion that the effects on growth of IL-1 and IFN-gamma are mediated by different mechanisms.
利用几种人类细胞系研究了重组人白细胞介素-1（IL-1）的α和β形式以及干扰素-γ（IFN-γ）的生长调节作用。将细胞暴露于IL-1和IFN-γ的组合中会产生三类细胞反应。第一类是IL-1刺激生长并抵消IFN-γ生长抑制作用的细胞系。这些细胞系包括肺癌细胞系CALU-1和结肠癌细胞系SW-48。第二类是对IL-1不敏感且仅被IFN-γ抑制的一些细胞系。这些包括宫颈癌细胞系HeLa、转化的乳腺细胞系HBL-100和髓性白血病细胞系K562。第三组由IL-1和IFN-γ的生长抑制作用具有相加性的细胞组成。这些包括乳腺癌细胞系MCF-7和MDA-MB-415。后一组的例外是ME-180，在其中无法证明显著的相加抑制作用。单独的IL-1主要在生长受抑制的细胞系中诱导细胞生长停滞效应。在存在IL-1的情况下，IFN-γ诱导的细胞溶解作用增强。这些数据支持以下结论：IL-1和IFN-γ对生长的影响是由不同机制介导的。
