Chikindas M L, Mironov V N, Luk'ianov E V, Boretskiĭ Iu R, Arutiunova L S
Mol Gen Mikrobiol Virusol. 1987 Apr(4):22-6.
All the structural genes of riboflavin biosynthesis are shown to be located on the 2.8 MD DNA fragment, using the collection of plasmids, carrying the Bacillus subtilis riboflavin operon fragments and Bacillus subtilis strains, containing various deletions of rib-operon for analysis. The proximal Bgl II site is shown to be located between promoter P1 and the first structural gene ribG. The distal Hind III site of fragment C is the left bound of the rib-operon.
利用携带枯草芽孢杆菌核黄素操纵子片段的质粒文库以及含有核黄素操纵子各种缺失的枯草芽孢杆菌菌株进行分析,结果表明核黄素生物合成的所有结构基因都位于2.8 MD的DNA片段上。近端Bgl II位点位于启动子P1和第一个结构基因ribG之间。片段C的远端Hind III位点是核黄素操纵子的左边界。