[通过质粒pLRS33整合到枯草芽孢杆菌染色体中诱导核黄素营养缺陷型突变]
[The induction of auxotrophic mutations for riboflavin by the integration of plasmid pLRS33 into the chromosome of Bacillus subtilis].
作者信息
Shevchenko T N, Timashova E O, Gorinchuk G F, Maliuta S S
出版信息
Genetika. 1988 Aug;24(8):1371-4.
PMID:3144477
Abstract
The transformation of Bacillus subtilis Lys- strains with plasmid pLRS33 containing pBR322 and the Bac. subtilis chromosomal fragment carrying the genes for lysin biosynthesis and the riboflavin operon regulatory operator region (ribO) leads to the appearance of Rib- mutants. It was shown that these mutants contained long deletions covering a great portion of the riboflavin operon.
摘要
用含有pBR322以及携带溶菌酶生物合成基因和核黄素操纵子调控操纵区（ribO）的枯草芽孢杆菌染色体片段的质粒pLRS33转化枯草芽孢杆菌Lys-菌株，会导致出现Rib-突变体。结果表明，这些突变体含有大片段缺失，覆盖了核黄素操纵子的很大一部分。
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