白细胞介素-1β预处理的脂肪组织来源间充质干细胞对血管内皮生长因子分泌及巨噬细胞M极化的影响

[Effects of IL-1β Pretreated Adipose Tissue-derived Mesenchymal Stem Cells on VEGF Secretion and Macrophage M Polarization].

作者信息

Liu Liu, Liu Lin, Liang Li-Chuan, Liu Dong-Liang, Zhu Zhi-Qiang

机构信息

Department of General Surgery, the First Affiliated Hospital of University of Science and Technology of China, Hefei 230001, China.

Department of Anesthesiology, the First Affiliated Hospital of University of Science and Technology of China, Hefei 230001, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2019 Mar;50(2):171-176.

PMID:31106534

Abstract

OBJECTIVE

To investigate the effects of interleukin (IL)-1β preteated adipose tissue-derived mesenchymal stem cells (ADMSCs) on vascular endothelial growth factor (VEGF) secretion and macrophages M polarization.

METHODS

After IL-1β pretreated ADMSC for 24 h, the expression of cyclooxygenase-2 (COX-2) was detected using Western blot, and the secretions of prostaglandin E (PGE) and VEGF were measured by ELISA method. Conditioned media collected from ADMSCs was used for culturing PMA-U937 macrophages for 72 h, and the expressions of and -10 mRNAs were detected using qRT-PCR. After inhibition of COX-2 expression in ADMSCs by Lentivirus silencing, secretion of VEGF and and -10 mRNA expressions were detected.

RESULTS

IL-1β pretreating ADMSCs increased the expression level of COX-2, enhanced PGE and VEGF secretions (<0.05). In addition, the mRNA levels of and -10 were upregulated when ADMSCs were pretreated by IL-1β in PMA-U937 cells. After downregulation of COX-2 in ADMSCs, the secretion of VEGF was suppressed, and the mRNA levels of and -10 were also significantly down-regulated.

CONCLUSION

IL-1β pretreatment enhanced the secretion of PGE and VEGF, and induced macrophage M polarization, which depended on COX-2-PGE signal pathway.

摘要

目的

探讨白细胞介素（IL）-1β预处理脂肪组织来源的间充质干细胞（ADMSC）对血管内皮生长因子（VEGF）分泌及巨噬细胞M极化的影响。

方法

IL-1β预处理ADMSC 24小时后，采用蛋白质免疫印迹法检测环氧化酶-2（COX-2）的表达，采用酶联免疫吸附测定法检测前列腺素E（PGE）和VEGF的分泌。收集ADMSC的条件培养基用于培养佛波酯诱导的U937巨噬细胞72小时，采用实时定量聚合酶链反应检测及 -10 mRNA的表达。慢病毒沉默ADMSC中COX-2表达后，检测VEGF的分泌及及 -10 mRNA的表达。